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2 results on '"H3K14ac"'

1. The Transcriptional Adaptor Protein ADA3a Modulates Flowering of Arabidopsis thaliana

Author

Konstantinos E. Vlachonasios, Despoina Dadarou, Stylianos Poulios, Amy T. Hark, John H. Doonan, Niki Mougiou, Vasileia Maliori, Nestoras Kargios, and Maxim Gavriilidis

Subjects
0106 biological sciences, 0301 basic medicine, Time Factors, SPL, Transcription, Genetic, Arabidopsis, 01 natural sciences, Histones, Gene Expression Regulation, Plant, Gene expression, Arabidopsis thaliana, lcsh:QH301-705.5, Phylogeny, biology, histone acetylation, Nuclear Proteins, food and beverages, Acetylation, General Medicine, H3K14Ac, Chromatin, Cell biology, Histone, Phenotype, Genome, Plant, Protein Binding, DNA, Bacterial, Flowers, ADA2, Article, ADA3, 03 medical and health sciences, Histone H3, FT, Protein Domains, flowering, Sequence Homology, Amino Acid, Arabidopsis Proteins, fungi, Wild type, Epistasis, Genetic, biology.organism_classification, 030104 developmental biology, lcsh:Biology (General), Mutation, biology.protein, gene expression, DELLA, Chromatin immunoprecipitation, 010606 plant biology & botany, GCN5
Abstract

Histone acetylation is directly related to gene expression. In yeast, the acetyltransferase general control nonderepressible-5 (GCN5) targets histone H3 and associates with transcriptional co-activators alteration/deficiency in activation-2 (ADA2) and alteration/deficiency in activation-3 (ADA3) in complexes like SAGA. Arabidopsis thaliana has two genes encoding proteins, designated ADA3a and ADA3b, that correspond to yeast ADA3. We investigated the role of ADA3a and ADA3b in regulating gene expression during flowering time. Specifically, we found that knock out mutants ada3a-2 and the double mutant ada3a-2 ada3b-2 lead to early flowering compared to the wild type plants under long day (LD) conditions and after moving plants from short days to LD. Consistent with ADA3a being a repressor of floral initiation, FLOWERING LOCUS T (FT) expression was increased in ada3a mutants. In contrast, other genes involved in multiple pathways leading to floral transition, including FT repressors, players in GA signaling, and members of the SPL transcriptional factors, displayed reduced expression. Chromatin immunoprecipitation analysis revealed that ADA3a affects the histone H3K14 acetylation levels in SPL3, SPL5, RGA, GAI, and SMZ loci. In conclusion, ADA3a is involved in floral induction through a GCN5-containing complex that acetylates histone H3 in the chromatin of flowering related genes.

Published
2021

2. The Transcriptional Adaptor Protein ADA3a Modulates Flowering of Arabidopsis thaliana .

Author

Poulios S, Dadarou D, Gavriilidis M, Mougiou N, Kargios N, Maliori V, Hark AT, Doonan JH, and Vlachonasios KE

Subjects
Acetylation, Arabidopsis genetics, Arabidopsis Proteins chemistry, Arabidopsis Proteins genetics, DNA, Bacterial genetics, Epistasis, Genetic, Flowers genetics, Gene Expression Regulation, Plant, Genome, Plant, Histones metabolism, Mutation genetics, Nuclear Proteins chemistry, Nuclear Proteins genetics, Phenotype, Phylogeny, Protein Binding, Protein Domains, Sequence Homology, Amino Acid, Time Factors, Arabidopsis metabolism, Arabidopsis Proteins metabolism, Flowers physiology, Nuclear Proteins metabolism, Transcription, Genetic
Abstract

Histone acetylation is directly related to gene expression. In yeast, the acetyltransferase general control nonderepressible-5 (GCN5) targets histone H3 and associates with transcriptional co-activators alteration/deficiency in activation-2 (ADA2) and alteration/deficiency in activation-3 (ADA3) in complexes like SAGA. Arabidopsis thaliana has two genes encoding proteins, designated ADA3a and ADA3b, that correspond to yeast ADA3. We investigated the role of ADA3a and ADA3b in regulating gene expression during flowering time. Specifically, we found that knock out mutants ada3a-2 and the double mutant ada3a-2 ada3b-2 lead to early flowering compared to the wild type plants under long day (LD) conditions and after moving plants from short days to LD. Consistent with ADA3a being a repressor of floral initiation, FLOWERING LOCUS T ( FT) expression was increased in ada3a mutants. In contrast, other genes involved in multiple pathways leading to floral transition, including FT repressors, players in GA signaling, and members of the SPL transcriptional factors, displayed reduced expression. Chromatin immunoprecipitation analysis revealed that ADA3a affects the histone H3K14 acetylation levels in SPL3 , SPL5 , RGA , GAI, and SMZ loci. In conclusion, ADA3a is involved in floral induction through a GCN5-containing complex that acetylates histone H3 in the chromatin of flowering related genes.

Published
2021
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