1. Improved human mesenchymal stem cell isolation.
- Author
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Chan TM, Harn HJ, Lin HP, Chou PW, Chen JY, Ho TJ, Chiou TW, Chuang HM, Chiu SC, Chen YC, Yen SY, Huang MH, Liang BC, and Lin SZ
- Subjects
- Adipose Tissue cytology, Bone Marrow Cells cytology, Cell Separation instrumentation, Humans, Mesenchymal Stem Cells metabolism, Cell Separation methods, Mesenchymal Stem Cells cytology
- Abstract
Human mesenchymal stem cells (hMSCs) are currently available for a range of applications and benefits and have become a good material for regenerative medicine, tissue engineering, and disease therapy. Before ex vivo expansion, isolation and characterization of primary hMSCs from peripheral tissues are key steps for obtaining adequate materials for clinical application. The proportion of peripheral stem cells is very low in surrounding tissues and organs; thus the recovery ratio will be a limiting factor. In this review, we summarized current common methods used to isolate peripheral stem cells, as well as the new insights revealed to improve the quantity of stem cells and their stemness. These strategies offer alternative ways to acquire hMSCs in a convenient and/or effective manner, which is important for clinical treatments. Improved isolation and mass amplification of the hMSCs while ensuring their stemness and quantity will be an important step for clinical use. Enlarged suitable hMSCs are more clinically applicable for therapeutic transplants and may help people live longer and better.
- Published
- 2014
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