1. CD157 Marks Tissue-Resident Endothelial Stem Cells with Homeostatic and Regenerative Properties
- Author
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Yoshihide Hayashizaki, Yang Lin, Hideya Kawaji, Hiroyasu Kidoya, Kohji Nishida, Mervin C. Yoder, Kazuhiro Takara, Jia Weizhen, Jun-ichi Suehiro, Masaaki Furuno, Taku Wakabayashi, Fumitaka Muramatsu, Tsukasa Kouno, Hisamichi Naito, Tomohiro Iba, Nobuyuki Takakura, Katsuhiko Ishihara, and Sachi Ishikawa-Kato
- Subjects
0301 basic medicine ,Pathology ,medicine.medical_specialty ,Stromal cell ,Angiogenesis ,Biology ,GPI-Linked Proteins ,Colony-Forming Units Assay ,03 medical and health sciences ,Antigens, CD ,Genetics ,medicine ,Animals ,Homeostasis ,Regeneration ,Cell Lineage ,ADP-ribosyl Cyclase ,Vein ,Endothelial Progenitor Cells ,Factor VIII ,Regeneration (biology) ,Cell Biology ,Mice, Inbred C57BL ,Transplantation ,Endothelial stem cell ,030104 developmental biology ,medicine.anatomical_structure ,Liver ,Blood Vessels ,Molecular Medicine ,Bone marrow ,Biomarkers ,Adult stem cell - Abstract
Summary The generation of new blood vessels via angiogenesis is critical for meeting tissue oxygen demands. A role for adult stem cells in this process remains unclear. Here, we identified CD157 (bst1, bone marrow stromal antigen 1) as a marker of tissue-resident vascular endothelial stem cells (VESCs) in large arteries and veins of numerous mouse organs. Single CD157 + VESCs form colonies in vitro and generate donor-derived portal vein, sinusoids, and central vein endothelial cells upon transplantation in the liver. In response to injury, VESCs expand and regenerate entire vasculature structures, supporting the existence of an endothelial hierarchy within blood vessels. Genetic lineage tracing revealed that VESCs maintain large vessels and sinusoids in the normal liver for more than a year, and transplantation of VESCs rescued bleeding phenotypes in a mouse model of hemophilia. Our findings show that tissue-resident VESCs display self-renewal capacity and that vascular regeneration potential exists in peripheral blood vessels.
- Published
- 2018
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