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Your search keyword '"Kelley, Douglas H."' showing total 3 results
Start Over Author "Kelley, Douglas H." Journal cell reports
3 results on '"Kelley, Douglas H."'

1. A real-time in vivo clearance assay for quantification of glymphatic efflux

Author

Plá, Virginia, primary, Bork, Peter, additional, Harnpramukkul, Aurakoch, additional, Olveda, Genaro, additional, Ladrón-de-Guevara, Antonio, additional, Giannetto, Michael J., additional, Hussain, Rashad, additional, Wang, Wei, additional, Kelley, Douglas H., additional, Hablitz, Lauren M., additional, and Nedergaard, Maiken, additional

Published
2022
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2. PDGF-B Is Required for Development of the Glymphatic System

Author

Munk, Anne Sofie, primary, Wang, Wei, additional, Bèchet, Nicholas Burdon, additional, Eltanahy, Ahmed M., additional, Cheng, Anne Xiaoan, additional, Sigurdsson, Björn, additional, Benraiss, Abdellatif, additional, Mäe, Maarja A., additional, Kress, Benjamin Travis, additional, Kelley, Douglas H., additional, Betsholtz, Christer, additional, Møllgård, Kjeld, additional, Meissner, Anja, additional, Nedergaard, Maiken, additional, and Lundgaard, Iben, additional

Published
2019
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3. A real-time in vivoclearance assay for quantification of glymphatic efflux

Author

Plá, Virginia, Bork, Peter, Harnpramukkul, Aurakoch, Olveda, Genaro, Ladrón-de-Guevara, Antonio, Giannetto, Michael J., Hussain, Rashad, Wang, Wei, Kelley, Douglas H., Hablitz, Lauren M., and Nedergaard, Maiken

Abstract

Glymphatic fluid transport eliminates metabolic waste from the brain including amyloid-β, yet the methodology for studying efflux remains rudimentary. Here, we develop a method to evaluate glymphatic real-time clearance. Efflux of Direct Blue 53 (DB53, also T-1824 or Evans Blue) injected into the striatum is quantified by imaging the DB53 signal in the vascular compartment, where it is retained due to its high affinity to albumin. The DB53 signal is detectable as early as 15 min after injection and the efflux kinetics are sharply reduced in mice lacking the water channel aquaporin 4 (AQP4). Pharmacokinetic modeling reveal that DB53 efflux is consistent with the existence of two efflux paths, one with fast kinetics (T1/2 = 50 min) and another with slow kinetics (T1/2 = 240 min), in wild-type mice. This in vivomethodology will aid in defining the physiological variables that drive efflux, as well as the impact of brain states or disorders on clearance kinetics.

Published
2022
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