1. Inter-dependent Centrosomal Co-localization of the cen and ik2 cis-Natural Antisense mRNAs in Drosophila.
- Author
-
Bergalet J, Patel D, Legendre F, Lapointe C, Benoit Bouvrette LP, Chin A, Blanchette M, Kwon E, and Lécuyer E
- Subjects
- Animals, Conserved Sequence, Drosophila Proteins genetics, Drosophila melanogaster embryology, Drosophila melanogaster genetics, Embryo, Nonmammalian metabolism, Evolution, Molecular, Gene Expression Regulation, Humans, I-kappa B Kinase genetics, Oocytes metabolism, Polyribosomes metabolism, Protein Biosynthesis, RNA Transport, RNA, Antisense genetics, RNA, Messenger genetics, RNA, Messenger metabolism, Centrosome metabolism, Drosophila Proteins metabolism, Drosophila melanogaster metabolism, I-kappa B Kinase metabolism, RNA, Antisense metabolism
- Abstract
Overlapping genes are prevalent in most genomes, but the extent to which this organization influences regulatory events operating at the post-transcriptional level remains unclear. Studying the cen and ik2 genes of Drosophila melanogaster, which are convergently transcribed as cis-natural antisense transcripts (cis-NATs) with overlapping 3' UTRs, we found that their encoded mRNAs strikingly co-localize to centrosomes. These transcripts physically interact in a 3' UTR-dependent manner, and the targeting of ik2 requires its 3' UTR sequence and the presence of cen mRNA, which serves as the main driver of centrosomal co-localization. The cen transcript undergoes localized translation in proximity to centrosomes, and its localization is perturbed by polysome-disrupting drugs. By interrogating global fractionation-sequencing datasets generated from Drosophila and human cellular models, we find that RNAs expressed as cis-NATs tend to co-localize to specific subcellular fractions. This work suggests that post-transcriptional interactions between RNAs with complementary sequences can dictate their localization fate in the cytoplasm., Competing Interests: Declaration of Interests The authors declare no competing interests., (Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2020
- Full Text
- View/download PDF