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Your search keyword '"Pei, Xuetao"' showing total 11 results
Start Over Author "Pei, Xuetao" Journal cell proliferation
11 results on '"Pei, Xuetao"'

1. Decoding human in vitro terminal erythropoiesis originating from umbilical cord blood mononuclear cells and pluripotent stem cells

Author

Wang, Xiaoling, primary, Zhang, Wei, additional, Zhao, Siqi, additional, Yan, Hao, additional, Xin, Zijuan, additional, Cui, Tiantian, additional, Zang, Ruge, additional, Zhao, Lingping, additional, Wang, Haiyang, additional, Zhou, Junnian, additional, Li, Xuan, additional, Yue, Wen, additional, Xi, Jiafei, additional, Zhang, Zhaojun, additional, Fang, Xiangdong, additional, and Pei, Xuetao, additional

Published
2024
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2. Baffled‐flow culture system enables the mass production of megakaryocytes from human embryonic stem cells by enhancing mitochondrial function.

Author

Wu, Xumin, Zhang, Bowen, Chen, Keyi, Zhao, Jiahui, Li, Yunxing, Li, Jisheng, Liu, Chuanli, He, Lijuan, Fan, Tao, Wang, Chao, Li, Yan, Pei, Xuetao, and Li, Yanhua

Subjects
HUMAN embryonic stem cells, MASS production, EMBRYONIC stem cells, MEGAKARYOCYTES, THROMBOPOIETIN receptors, AUTOMATION, MITOCHONDRIA, CELL culture
Abstract

Human embryonic stem cells (hESCs) have become an ideal cell source for the ex vivo generation of megakaryocyte (MK) and platelet products for clinical applications. However, an ongoing challenge is to establish scalable culture systems to maximize the yield of stem cell‐derived MKs that release platelets. We defined a specific dynamic 3D manufacturing system in a baffled‐flow manner that could remarkably facilitate megakaryopoiesis and increase the yield of platelet‐producing MKs from hESCs within a 12‐day induction period. Additionally, an increased number of >16N ploidy MKs, proplatelets, and platelets were generated from induced cells harvested on Day 12 using the specific dynamic culture method. The specific dynamic culture method significantly enhanced endothelium‐to‐haematopoietic transition and early haematopoiesis. More importantly, MK fate was significantly facilitated in a specific dynamic manner during early haematopoiesis. Mechanistically, this dynamic culture significantly enhanced mitochondrial function via the oxidative phosphorylation pathway and caused differentiation skewing of hESCs toward megakaryopoiesis. This study can aid in the automatic and scalable production of MKs from stem cells using baffled‐flow bioreactors and assist in the manufacturing of hESC‐derived MK and platelet products. [ABSTRACT FROM AUTHOR]

Published
2023
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3. Generation of Rh D‐negative blood using CRISPR / Cas9

Author

Xu, Lei, primary, Zeng, Quan, additional, Liang, Liqing, additional, Yang, Zhou, additional, Qu, Mingyi, additional, Li, Huilin, additional, Zhang, Bowen, additional, Zhang, Jing, additional, Yuan, Xin, additional, Chen, Lin, additional, Fan, Zeng, additional, He, Lijuan, additional, Nan, Xue, additional, Yue, Wen, additional, Xie, Xiaoyan, additional, and Pei, Xuetao, additional

Published
2023
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5. Requirements for human haematopoietic stem/progenitor cells

Author

Nan, Xue, primary, Zhang, Bowen, additional, Hao, Jie, additional, Yue, Wen, additional, Fu, Boqiang, additional, Qu, Mingyi, additional, Zhang, Yu, additional, Wang, Haiyang, additional, Fang, Fang, additional, Wei, Jun, additional, Li, Qiyuan, additional, Hu, Shijun, additional, Yu, Junying, additional, Gao, Yingdai, additional, Liu, Qifa, additional, Cao, Jiani, additional, Wang, Lei, additional, Peng, Yaojin, additional, Zhu, Huanxin, additional, Liang, Lingmin, additional, Ma, Aijin, additional, Zhou, Jiaxi, additional, Zhao, Tongbiao, additional, and Pei, Xuetao, additional

Published
2021
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6. Requirements for human cardiomyocytes

Author

Yu, Miao, primary, Lei, Wei, additional, Cao, Jiani, additional, Wang, Lei, additional, Ma, Aijin, additional, Zhao, Zhen‐Ao, additional, Yang, Huang‐Tian, additional, Shen, Zhenya, additional, Lan, Feng, additional, Cao, Feng, additional, Liang, Ping, additional, Pei, Xuetao, additional, Xiang, Andy Peng, additional, Yu, Junying, additional, Zhang, Yu, additional, Zhang, Yong, additional, Li, Qiyuan, additional, Zhou, Jiaxi, additional, Wei, Jun, additional, Peng, Yaojin, additional, Zhu, Huanxin, additional, Liang, Lingmin, additional, Cao, Nan, additional, Fu, Boqiang, additional, Hao, Jie, additional, Zhao, Tongbiao, additional, and Hu, Shijun, additional

Published
2021
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7. Large‐scale generation of megakaryocytes from human embryonic stem cells using transgene‐free and stepwise defined suspension culture conditions

Author

Zhang, Bowen, primary, Wu, Xumin, additional, Zi, Guicheng, additional, He, Lijuan, additional, Wang, Sihan, additional, Chen, Lin, additional, Fan, Zeng, additional, Nan, Xue, additional, Xi, Jiafei, additional, Yue, Wen, additional, Wang, Lei, additional, Wang, Liu, additional, Hao, Jie, additional, Pei, Xuetao, additional, and Li, Yanhua, additional

Published
2021
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8. Requirements for human cardiomyocytes.

Author

Yu, Miao, Lei, Wei, Cao, Jiani, Wang, Lei, Ma, Aijin, Zhao, Zhen‐Ao, Yang, Huang‐Tian, Shen, Zhenya, Lan, Feng, Cao, Feng, Liang, Ping, Pei, Xuetao, Xiang, Andy Peng, Yu, Junying, Zhang, Yu, Zhang, Yong, Li, Qiyuan, Zhou, Jiaxi, Wei, Jun, and Peng, Yaojin

Subjects
STANDARDIZATION, STEM cell research, HUMAN beings
Abstract

'Requirements for human cardiomyocytes', jointly drafted and agreed upon by experts from the Chinese Society for Stem Cell Research, is the first guideline for human cardiomyocytes in China. This standard specifies the technical requirements, test methods, test regulations, instructions for use, labelling requirements, packing requirements, storage requirements, transportation requirements and waste disposal requirements for human cardiomyocytes, which is designed to normalize and standardize human cardiomyocyte research and production. It was originally released by the China Society for Cell Biology on 9 January 2021. We hope that the publication of this guideline will promote institutional establishment, acceptance and execution of proper protocols, and accelerate the international standardization of human cardiomyocytes for applications. [ABSTRACT FROM AUTHOR]

Published
2022
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9. General requirements for stem cells

Author

Hao, Jie, primary, Ma, Aijin, additional, Wang, Lei, additional, Cao, Jiani, additional, Chen, Si, additional, Wang, Liu, additional, Fu, Boqiang, additional, Zhou, Jiaxi, additional, Pei, Xuetao, additional, Zhang, Yu, additional, Xiang, Peng, additional, Hu, Shijun, additional, Li, Qiyuan, additional, Zhang, Yong, additional, Xia, Yingjie, additional, Zhu, Huanxin, additional, Stacey, Glyn, additional, Zhou, Qi, additional, and Zhao, Tongbiao, additional

Published
2020
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10. Requirements for human embryonic stem cells

Author

Hao, Jie, primary, Cao, Jiani, additional, Wang, Lei, additional, Ma, Aijin, additional, Chen, Si, additional, Ding, Jinfeng, additional, Wang, Liu, additional, Fu, Boqiang, additional, Zhang, Yu, additional, Pei, Xuetao, additional, Xiang, Peng, additional, Li, Qiyuan, additional, Zhang, Yong, additional, Zhou, Jiaxi, additional, Hu, Shijun, additional, Yu, Junying, additional, Wei, Jun, additional, Zhu, Huanxin, additional, Stacey, Glyn, additional, Zhao, Tongbiao, additional, and Zhou, Qi, additional

Published
2020
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11. Requirements for human haematopoietic stem/progenitor cells.

Author

Nan X, Zhang B, Hao J, Yue W, Fu B, Qu M, Zhang Y, Wang H, Fang F, Wei J, Li Q, Hu S, Yu J, Gao Y, Liu Q, Cao J, Wang L, Peng Y, Zhu H, Liang L, Ma A, Zhou J, Zhao T, and Pei X

Subjects
China, Humans, Hematopoietic Stem Cells
Abstract

'Requirements for human haematopoietic stem/progenitor cells' is the first set of guidelines on human haematopoietic stem/progenitor cells in China, jointly drafted and agreed upon by experts from the Chinese Society for Stem Cell Research. This standard specifies the technical requirements, inspection methods, inspection rules, instructions for usage, labelling requirements, packaging requirements, storage requirements and transportation requirements for human haematopoietic stem/progenitor cells, which is applicable to the quality control for human haematopoietic stem/progenitor cells. We hope that publication of these guidelines will promote institutional establishment, acceptance and execution of proper protocols, and accelerate the international standardization of human haematopoietic stem/progenitor cells for applications., (© 2021 The Authors. Cell Proliferation published by John Wiley & Sons Ltd.)

Published
2022
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