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Your search keyword '"Jian, Ruiqi"' showing total 2 results
Start Over Author "Jian, Ruiqi" Journal cell host & microbe
2 results on '"Jian, Ruiqi"'

1. Longitudinal profiling of the microbiome at four body sites reveals core stability and individualized dynamics during health and disease

Author

Zhou, Xin, Shen, Xiaotao, Johnson, Jethro S., Spakowicz, Daniel J., Agnello, Melissa, Zhou, Wenyu, Avina, Monica, Honkala, Alexander, Chleilat, Faye, Chen, Shirley Jingyi, Cha, Kexin, Leopold, Shana, Zhu, Chenchen, Chen, Lei, Lyu, Lin, Hornburg, Daniel, Wu, Si, Zhang, Xinyue, Jiang, Chao, Jiang, Liuyiqi, Jiang, Lihua, Jian, Ruiqi, Brooks, Andrew W., Wang, Meng, Contrepois, Kévin, Gao, Peng, Rose, Sophia Miryam Schüssler-Fiorenza, Tran, Thi Dong Binh, Nguyen, Hoan, Celli, Alessandra, Hong, Bo-Young, Bautista, Eddy J., Dorsett, Yair, Kavathas, Paula B., Zhou, Yanjiao, Sodergren, Erica, Weinstock, George M., and Snyder, Michael P.

Published
2024
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2. Systematic Identification of Host Cell Regulators of Legionella pneumophila Pathogenesis Using a Genome-wide CRISPR Screen

Author

Jeng, Edwin E, Bhadkamkar, Varun, Ibe, Nnejiuwa U, Gause, Haley, Jiang, Lihua, Chan, Joanne, Jian, Ruiqi, Jimenez-Morales, David, Stevenson, Erica, Krogan, Nevan J, Swaney, Danielle L, Snyder, Michael P, Mukherjee, Shaeri, and Bassik, Michael C

Subjects
Biochemistry and Cell Biology, Biological Sciences, Pneumonia & Influenza, Lung, Infectious Diseases, Pneumonia, Aetiology, 2.1 Biological and endogenous factors, Infection, Animals, Bacterial Proteins, Cell Line, Clustered Regularly Interspaced Short Palindromic Repeats, Endoplasmic Reticulum, Guanine Nucleotide Exchange Factors, HEK293 Cells, HeLa Cells, Humans, Legionella pneumophila, Legionnaires' Disease, Macrophages, Membrane Proteins, Mice, Phagocytosis, Proteins, RAW 264.7 Cells, U937 Cells, Vacuoles, Virulence Factors, rab GTP-Binding Proteins, Hela Cells, C1ORF43, CRISPR screen, RAB10, host-pathogen interaction, intracellular bacteria, pathogen, phagocytosis, Microbiology, Medical Microbiology, Immunology, Biochemistry and cell biology, Medical microbiology
Abstract

During infection, Legionella pneumophila translocates over 300 effector proteins into the host cytosol, allowing the pathogen to establish an endoplasmic reticulum (ER)-like Legionella-containing vacuole (LCV) that supports bacterial replication. Here, we perform a genome-wide CRISPR-Cas9 screen and secondary targeted screens in U937 human monocyte/macrophage-like cells to systematically identify host factors that regulate killing by L. pneumophila. The screens reveal known host factors hijacked by L. pneumophila, as well as genes spanning diverse trafficking and signaling pathways previously not linked to L. pneumophila pathogenesis. We further characterize C1orf43 and KIAA1109 as regulators of phagocytosis and show that RAB10 and its chaperone RABIF are required for optimal L. pneumophila replication and ER recruitment to the LCV. Finally, we show that Rab10 protein is recruited to the LCV and ubiquitinated by the effectors SidC/SdcA. Collectively, our results provide a wealth of previously undescribed insights into L. pneumophila pathogenesis and mammalian cell function.

Published
2019

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