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We have isolated the chicken beta-type globin genes from a library of chicken DNA--lambda Charon 4A recombinant bacteriophage. There are four beta-type genes within this segment of the genome; we believe this represents all of the beta-type genes of the chicken. The recombinant lambda C beta G1 contains the embryonic epsilon- and adult beta-globin genes. The hatching beta H- and embryonic rho-globin genes are found in the recombinant lambda C beta G2. Although lambda C beta G1 and lambda C beta G2 do not physically overlap, we present evidence that all four genes are closely linked and transcribed from the same DNA strand. These experiments demonstrate that the chromosomal regions represented by lambda C beta G1 and lambda C beta G2 lie approximately 1.6 kb apart in the chicken genome. A third recombinant lambda C beta G3 extends the genomic locus studied in the vicinity of the beta-type globin genes to approximately 39 kb. The physical order of the chicken beta-type globin genes within this segment of the chromosome is 5' ... rho--beta H--beta--epsilon ... 3'. This arrangement is unique among the vertebrate beta-type globin gene clusters thus far examined, in that embryonic genes are located at the 5' and 3' ends of the cluster while the hatching and adult genes occupy central positions.