1. Globin RNA transcription: A possible termination site and demonstration of transcriptional control correlated with altered chromatin structure
- Author
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Erhard Hofer, Renate Hofer-Warbinek, and James E. Darnell
- Subjects
RNA Caps ,Messenger RNA ,Deoxyribonucleases ,Transcription, Genetic ,General transcription factor ,RNA ,Biology ,Molecular biology ,Chromatin ,General Biochemistry, Genetics and Molecular Biology ,Globins ,Mice ,genomic DNA ,Gene Expression Regulation ,Transcription (biology) ,hemic and lymphatic diseases ,Transcriptional regulation ,Animals ,Dimethyl Sulfoxide ,RNA, Messenger ,Globin ,Cloning, Molecular - Abstract
The transcription unit that encodes beta-globin (major) mRNA has been examined by analysis of nascent labeled RNA from the nuclei of mouse erythroleukemia cells treated with agents that induce beta-globin formation. More than 95% of the time, transcription appears to terminate within a region 1400 +/- 100 nucleotides downstream from the poly(A) site. The nuclei of mouse erythroleukemia cells treated with agents that induce beta-globin synthesis showed a 10 to 20 fold stimulation of transcription assayed by chain elongation of beta-globin RNA sequences, including sequences downstream from the poly(A) site. Associated with the increase in transcription was a generalized increase in sensitivity to DNAase treatment of globin genomic DNA in whole nuclei. A DNAase I-hypersensitive site in the vicinity of the cap site was also found to be prominent in induced cells.
- Published
- 1982
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