1. Kid-Mediated Chromosome Compaction Ensures Proper Nuclear Envelope Formation
- Author
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Reiko Horai, Kenjiro Adachi, Miho Ohsugi, Yoichiro Iwakura, Tadashi Yamamoto, Katsuko Sudo, Hayato Kotaki, Noriko Tokai-Nishizumi, Hiroshi Sagara, and Shigeru Kakuta
- Subjects
Male ,Blastomeres ,Somatic cell ,Nuclear Envelope ,PROTEINS ,education ,Kinesins ,DEVBIO ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Mice ,Multinucleate ,Animals ,Humans ,Telophase ,Mitosis ,Crosses, Genetic ,Anaphase ,Genetics ,Biochemistry, Genetics and Molecular Biology(all) ,Chromosome ,Embryo, Mammalian ,Embryonic stem cell ,Chromosomes, Mammalian ,humanities ,Cell biology ,DNA-Binding Proteins ,Anaphase lag ,Female ,CELLBIO ,HeLa Cells - Abstract
SummaryToward the end of mitosis, neighboring chromosomes gather closely to form a compact cluster. This is important for reassembling the nuclear envelope around the entire chromosome mass but not individual chromosomes. By analyzing mice and cultured cells lacking the expression of chromokinesin Kid/kinesin-10, we show that Kid localizes to the boundaries of anaphase and telophase chromosomes and contributes to the shortening of the anaphase chromosome mass along the spindle axis. Loss of Kid-mediated anaphase chromosome compaction often causes the formation of multinucleated cells, specifically at oocyte meiosis II and the first couple of mitoses leading to embryonic death. In contrast, neither male meiosis nor somatic mitosis after the morula-stage is affected by Kid deficiency. These data suggest that Kid-mediated anaphase/telophase chromosome compaction prevents formation of multinucleated cells. This protection is especially important during the very early stages of development, when the embryonic cells are rich in ooplasm.
- Published
- 2008
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