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The hydroisomerization of n-alkanes is an important step in the production of high-quality bio-aviation kerosene. A SAPO-11 molecular sieve was synthesized using the hydrothermal synthesis method, and a 0.5 wt% Pt/SAPO-11 catalyst was prepared using the impregnation method. The crystal phase, pore structure, acidity, and morphology of Pt/SAPO-11 were characterized via X-ray diffraction, N2 adsorption-desorption, NH3 temperature-programmed desorption, scanning electron microscopy, and transmission electron microscopy, respectively. The hydroisomerization performance of the catalyst was evaluated with bio-n-hexadecane as the model compound. The results showed that temperature and space velocity had significant effects. Under the conditions of 340 °C, 1.5 MPa, WHSV = 1.0 h−1, V(H2): V(n-hexadecane) = 1000:1, the conversion of n-hexadecane and the selectivity of i-hexadecane were 81.8% and 86.5%, respectively. [ABSTRACT FROM AUTHOR]