1. The structure of O-polysaccharides isolated from plant pathogenic bacteria Pectobacterium wasabiae IFB5408 and IFB5427
- Author
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Karolina Ossowska, Zbigniew Kaczyński, Małgorzata Czerwicka, Sabina Zoledowska, Wojciech Sledz, Agata Motyka, Sylwia Szulta, and Ewa Lojkowska
- Subjects
0106 biological sciences ,0301 basic medicine ,Magnetic Resonance Spectroscopy ,Stereochemistry ,Chemical structure ,Pectobacterium wasabiae ,Pectobacterium ,medicine.disease_cause ,Polysaccharide ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,Microbiology ,03 medical and health sciences ,Polysaccharides ,medicine ,Carbohydrate Conformation ,Monosaccharide ,Plant Diseases ,chemistry.chemical_classification ,biology ,Chemistry ,Organic Chemistry ,Pathogenic bacteria ,General Medicine ,Nuclear magnetic resonance spectroscopy ,Reference Standards ,biology.organism_classification ,030104 developmental biology ,Two-dimensional nuclear magnetic resonance spectroscopy ,Heteronuclear single quantum coherence spectroscopy ,010606 plant biology & botany - Abstract
O-Polysaccharides were isolated from the lipopolysaccharides of two strains of plant pathogenic bacteria Pectobacterium wasabiae isolated in Poland in 2013 (IFB5408 and IFB5427). The purified polysaccharides were analyzed using 1D and 2D NMR spectroscopy ((1)H, DQF-COSY, TOCSY, ROESY, HSQC, HSQC-TOCSY, and HMBC) and the chemical methods. Sugar and methylation analyses of native polysaccharides, absolute configuration assignment of constituent monosaccharides together with NMR spectroscopy data revealed that the chemical structures of both O-polysaccharides are the same.
- Published
- 2016