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Your search keyword '"Bibhas K. Bhunia"' showing total 5 results
Start Over Author "Bibhas K. Bhunia" Journal carbohydrate research
5 results on '"Bibhas K. Bhunia"'

1. A (1→6)-β-glucan from a somatic hybrid of Pleurotus florida and Volvariella volvacea: isolation, characterization, and study of immunoenhancing properties

Author

Bibhas K. Bhunia, Subhas Mondal, Debsankar Das, Debabrata Maiti, Sadhan K. Roy, Syed S. Islam, Tapas K. Maiti, and Samir R. Sikdar

Subjects
Magnetic Resonance Spectroscopy, Hybrid Cells, Pleurotus, Polysaccharide, Biochemistry, Beta-glucan, Volvariella, Analytical Chemistry, Cell Fusion, Mice, chemistry.chemical_compound, Animals, Immunologic Factors, Glucans, Cell Proliferation, Glucan, chemistry.chemical_classification, biology, Protoplasts, Organic Chemistry, Volvariella volvacea, General Medicine, Protoplast, biology.organism_classification, Somatic fusion, chemistry, Acid hydrolysis, Spleen
Abstract

A water-soluble immunoenhancing polysaccharide was isolated from the aqueous extract of fruit bodies of somatic hybrid (Pflo Vv5 FB), obtained through protoplast fusion between Pleurotus florida and Volvariella volvacea strains. On the basis of acid hydrolysis, the polysaccharide was found to contain glucose only. Methylation analysis, periodate oxidation along with 1H, DEPT-135, and 13C NMR spectroscopy, including two-dimensional TOCSY, DQF-COSY, NOESY, ROESY, 1H,13C-HMQC, and HMBC experiments showed that the polysaccharide was a (1→6)-β- d -glucan, which was not a constituent of any of the parent mushrooms previously reported. This glucan stimulated the macrophages, splenocytes, and thymocytes.

Published
2010

2. Isolation and characterization of a heteropolysaccharide from the corm of Amorphophallus campanulatus

Author

Tapas K. Maiti, Debabrata Maiti, Syed S. Islam, Sadhan K. Roy, Bibhas K. Bhunia, Debsankar Das, and Subhas Mondal

Subjects
Arabinose, Magnetic Resonance Spectroscopy, Stereochemistry, Chemical structure, Corm, Polysaccharide, Biochemistry, Analytical Chemistry, Mice, chemistry.chemical_compound, Amorphophallus, Polysaccharides, Animals, Organic chemistry, chemistry.chemical_classification, biology, Plant Extracts, Chemistry, Monosaccharides, Organic Chemistry, General Medicine, Nuclear magnetic resonance spectroscopy, biology.organism_classification, Carbohydrate Sequence, Solubility, Galactose, Acid hydrolysis, Sequence Analysis, Spleen
Abstract

A water-soluble polysaccharide isolated from the aqueous extract of the corm of Amorphophallus campanulatus was found to contain d -galactose, d -glucose, 4-O-acyl- d -methyl galacturonate, and l -arabinose in a molar ratio 2:1:1:1. Structural investigation of the polysaccharide was carried out using acid hydrolysis, methylation analysis, periodate oxidation study, and NMR studies (1H, 13C, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC). On the basis of the above-mentioned experiments the structure of the repeating unit of the polysaccharide was established as: This molecule showed splenocyte activation.

Published
2009

3. Structural studies of an immunoenhancing water-soluble glucan isolated from hot water extract of an edible mushroom, Pleurotus florida, cultivar Assam Florida

Author

Bibhas K. Bhunia, Tapas K. Maiti, Subhas Mondal, Syed S. Islam, Debsankar Das, Debabrata Maiti, and Sadhan K. Roy

Subjects
Thymus Gland, Polysaccharide, Biochemistry, Analytical Chemistry, Mice, Hydrolysis, chemistry.chemical_compound, Botany, Animals, Monosaccharide, Fruiting Bodies, Fungal, Food science, Cultivar, Glucans, Glucan, chemistry.chemical_classification, Pleurotus, Plants, Medicinal, biology, Chemistry, Macrophages, Organic Chemistry, Water, Periodate, General Medicine, Macrophage Activation, biology.organism_classification, Edible mushroom, Glucose, Solubility, Agaricales, Spleen
Abstract

An immunoenhancing polysaccharide isolated from the hot water extract of the fruiting bodies of an edible mushroom Pleurotus florida, cultivar Assam Florida, was found to consist of only d-glucose as a monosaccharide constituent. On the basis of total acid hydrolysis, methylation analysis, periodate oxidation, Smith degradation, and NMR experiments ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC), the structure of the repeating unit of the polysaccharide was established as This glucan stimulates macrophages, splenocytes, and thymocytes.

Published
2009

4. Isolation and characterization of the immunostimulating β-glucans of an edible mushroom Termitomyces robustus var

Author

Sunil K. Bhanja, Syed S. Islam, Chanchal K. Nandan, Tapas K. Maiti, Soumitra Mandal, Bibhas K. Bhunia, and Soumitra Mondal

Subjects
beta-Glucans, Termitomyces robustus, Polysaccharide, Nitric Oxide, Biochemistry, Analytical Chemistry, Immunomodulation, Hydrolysis, chemistry.chemical_compound, Mice, Splenocyte, Animals, Chemical Precipitation, Food science, Fruiting Bodies, Fungal, Cells, Cultured, Cell Proliferation, chemistry.chemical_classification, Thymocytes, Ethanol, Organic Chemistry, Periodate, Termitomyces, General Medicine, Nuclear magnetic resonance spectroscopy, Edible mushroom, chemistry, Macrophages, Peritoneal, Heteronuclear single quantum coherence spectroscopy, Spleen
Abstract

Two immunostimulating β-glucans, PS-I (water soluble) and PS-II (water insoluble) isolated from hot water extract of the fruiting bodies of an edible mushroom Termitomyces robustus var. showed significant macrophage, splenocyte, and thymocyte activation. On the basis of total hydrolysis, methylation analysis, periodate oxidation, and NMR experiments ((1)H, (13)C, DQF-COSY, TOCSY, DEPT-135, HSQC, and HMBC), the structure of the repeating unit of the polysaccharides is established as: PS-I: -6)β-D-Glcp-(1→ (Water-soluble glucan) PS-II: →3)-β-D-Glcp-(1→3)-β-D-Glcp-(1→ 6↑1 β-D-Glcp (Water-insoluble glucan, Termitan).

Published
2012

5. Structural analysis of an immunoenhancing heteropolysaccharide isolated from the green (unripe) fruits of Solenum melongena (Brinjal)

Author

Kaushik Ghosh, Krishnendu Chandra, Bibhas K. Bhunia, Tapas K. Maiti, Arnab K. Ojha, and Syed S. Islam

Subjects
chemistry.chemical_classification, Arabinose, Stereochemistry, Chemical structure, Organic Chemistry, Molecular Sequence Data, Periodate, General Medicine, Nuclear magnetic resonance spectroscopy, Polysaccharide, Biochemistry, Analytical Chemistry, chemistry.chemical_compound, Hydrolysis, chemistry, Carbohydrate Sequence, Polysaccharides, Galactose, Fruit, Organic chemistry, Solanum melongena, Two-dimensional nuclear magnetic resonance spectroscopy, Nuclear Magnetic Resonance, Biomolecular
Abstract

A water-soluble polysaccharide (SMPS) isolated from the aqueous extract of the green (unripe) fruits of Solenum melongena (Brinjal) contains D-galactose, D-methyl galacturonate, 3-O-acetyl D-methyl galacturonate, and L-arabinose in a molar proportion of nearly 1:1:1:1. Structural investigation of the polysaccharide was carried out using total acid hydrolysis, methylation analysis, periodate oxidation study, and NMR studies ((1)H, (13)C, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC). On the basis of the above experiments the structure of the repeating unit of the polysaccharide (SMPS) was established as --5)-alpha-L-Araf-(1--4)-beta-D-Galp-(1--2)-alpha-D-3-O-Ac-GalpA6Me-(1--2)alpha-D-galpA6Me-(1--This molecule showed splenocyte and thymocyte activations.

Published
2009

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