1. Development of a rapid method for the quantification of cellulose in tobacco by 13 C CP/MAS NMR
- Author
-
Chen Kaibo, Jiang Jinhui, Hu Yonghua, Jun Yang, Tian Zhenfeng, Shaolin Ge, Dong Tian, and Xu Yingbo
- Subjects
Magnetic Resonance Spectroscopy ,Polymers and Plastics ,Silicon ,Calibration curve ,Organic Chemistry ,Analytical chemistry ,chemistry.chemical_element ,Resonance ,02 engineering and technology ,Nuclear magnetic resonance spectroscopy ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Spectral line ,0104 chemical sciences ,chemistry.chemical_compound ,chemistry ,Tobacco ,Materials Chemistry ,Magic angle spinning ,Deconvolution ,Cellulose ,0210 nano-technology - Abstract
A method was developed for rapid quantitative determination of cellulose in tobacco by utilizing (13)C cross polarization magic angle spinning NMR spectroscopy ((13)C CP/MAS NMR). Sample powder was loaded into NMR rotor, which was customized rotor containing a matched silicon tube as an intensity reference. (13)C CP/MAS NMR spectra of tobacco samples were processed with spectral deconvolution to obtain the area of the C-1 resonance at 105.5ppm and the internal standard at 0ppm. The ratio between the area of 105.5ppm and 0ppm of a set of standard cellulose samples was used to construct a calibration curve. The cellulose content of a tobacco sample was determined by comparison of the ratio between the area of 105.5ppm and 0ppm to the calibration curve. Results of this developed method showed good agreement with those obtained from chemical analysis. The proposed method has such advantages of accuracy, quickness and efficiency, and could be an alternative to chemical analyses of cellulose.
- Published
- 2016