1. Chitosan-based hydrogel implants enriched with calcium ions intended for peripheral nervous tissue regeneration.
- Author
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Nawrotek K, Tylman M, Rudnicka K, Balcerzak J, and Kamiński K
- Subjects
- Animals, Biocompatible Materials adverse effects, Cell Line, Cell Line, Tumor, Cell Survival drug effects, Humans, Hydrogels adverse effects, Mice, Tissue Engineering instrumentation, Tissue Scaffolds adverse effects, Biocompatible Materials chemistry, Calcium chemistry, Chitosan chemistry, Hydrogels chemistry, Nerve Regeneration, Tissue Scaffolds chemistry
- Abstract
A new method for fabrication of chitosan-based hydrogel implants intended for peripheral nervous tissue regeneration was developed. The method is based on an electrodeposition phenomenon from a solution of chitosan and organic acid. In order to increase the mechanical strength of the implant, the solution was enriched with hydroxyapatite. Hydroxyapatite served as a source of calcium ions too. The influence of the concentration of the polymer and the additive on chemical, mechanical as well as biological properties of the obtained implant was evaluated. The study showed great dependence of the initial solution composition mainly on the physicochemical properties of the resulting structure. Basic in vitro cytotoxic and pro-inflammatory assays showed biocompatibility of manufactured implants, therefore, animal experimentations may be considered., (Copyright © 2015 Elsevier Ltd. All rights reserved.)
- Published
- 2016
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