13 results on '"Ernst Jan"'
Search Results
2. DNA and RNA Alterations Associated with Colorectal Peritoneal Metastases: A Systematic Review
- Author
-
Danique J. I. Heuvelings, Anne G. W. E. Wintjens, Julien Luyten, Guus E. W. A. Wilmink, Laura Moonen, Ernst-Jan M. Speel, Ignace H. J. T. de Hingh, Nicole D. Bouvy, and Andrea Peeters
- Subjects
Cancer Research ,PROGNOSIS ,MICROSATELLITE INSTABILITY ,ORIGIN ,IMPACT ,CARCINOMATOSIS ,biomarkers ,colorectal cancer ,genetic mutations ,CANCER ,LIVER METASTASES ,peritoneal metastases ,Oncology ,systematic review ,BRAF MUTATION ,MARKER ,RAS - Abstract
Simple Summary Colorectal cancer (CRC) patients with peritoneal metastases (PM) have a poor prognosis. Currently, research has been ongoing to develop new treatment options for CRC patients with PM. DNA/RNA alterations identification in the primary tumor might help identify patients who are at high risk for developing PM postoperatively. These patients could benefit from preventive or early treatment. The aim of this systematic review is to create an overview of studies which analyzed genomic DNA and RNA expression alteration correlated to PM with the goal of identifying potentially predictive biomarkers. We included 32 studies investigating primary colorectal tumors of 18,906 patients. Only BRAF mutations were reported as significantly associated with PM in 10 of 17 studies. As no specific biomarkers in the primary tumors of CRC patients could have been identified, further research with comprehensive genomic profiling is still desirable. Background: As colorectal cancer (CRC) patients with peritoneal metastases (PM) have a poor prognosis, new treatment options are currently being investigated for CRC patients. Specific biomarkers in the primary tumor could serve as a prediction tool to estimate the risk of distant metastatic spread. This would help identify patients eligible for early treatment. Aim: To give an overview of previously studied DNA and RNA alterations in the primary tumor correlated to colorectal PM and investigate which gene mutations should be further studied. Methods: A systematic review of all published studies reporting genomic analyses on the primary tissue of CRC tumors in relation to PM was undertaken according to PRISMA guidelines. Results: Overall, 32 studies with 18,906 patients were included. BRAF mutations were analyzed in 17 articles, of which 10 found a significant association with PM. For all other reported genes, no association with PM was found. Two analyses with broader cancer panels did not reveal any new biomarkers. Conclusion: An association of specific biomarkers in the primary tumors of CRC patients with metastatic spread into peritoneum could not be proven. The role of BRAF mutations should be further investigated. In addition, studies searching for potential novel biomarkers are still required.
- Published
- 2023
3. Evaluation Criteria for Chromosome Instability Detection by FISH to Predict Malignant Progression in Premalignant Glottic Laryngeal Lesions
- Author
-
Bergshoeff, Verona E., primary, Balkenhol, Maschenka C. A., additional, Haesevoets, Annick, additional, Ruland, Andrea, additional, Chenault, Michelene N., additional, Nelissen, Rik C., additional, Peutz, Carine J., additional, Clarijs, Ruud, additional, Van der Laak, Jeroen A. W. M. Van der, additional, Takes, Robert P., additional, Van den Brekel, Michiel W. Van den, additional, Van Velthuysen, Marie-Louise F. Van, additional, Ramaekers, Frans C. S., additional, Kremer, Bernd, additional, and Speel, Ernst-Jan M., additional
- Published
- 2022
- Full Text
- View/download PDF
4. Upfront Surgery vs. Primary Chemoradiation in an Unselected, Bicentric Patient Cohort with Oropharyngeal Squamous Cell Carcinoma—A Matched-Pair Analysis
- Author
-
Zimmermann, Philipp H., primary, Stuut, Marijn, additional, Wuerdemann, Nora, additional, Möllenhoff, Kathrin, additional, Suchan, Malte, additional, Eckel, Hans, additional, Wolber, Philipp, additional, Sharma, Shachi J., additional, Kämmerer, Fabian, additional, Langer, Christine, additional, Wittekindt, Claus, additional, Wagner, Steffen, additional, Kremer, Bernd, additional, Speel, Ernst Jan M., additional, and Klussmann, Jens P., additional
- Published
- 2021
- Full Text
- View/download PDF
5. Causes and Consequences of HPV Integration in Head and Neck Squamous Cell Carcinomas: State of the Art
- Author
-
Balaji, Harini, primary, Demers, Imke, additional, Wuerdemann, Nora, additional, Schrijnder, Julia, additional, Kremer, Bernd, additional, Klussmann, Jens Peter, additional, Huebbers, Christian Ulrich, additional, and Speel, Ernst-Jan Maria, additional
- Published
- 2021
- Full Text
- View/download PDF
6. Prognostic and Predictive Value of Integrated Qualitative and Quantitative Magnetic Resonance Imaging Analysis in Glioblastoma
- Author
-
Verduin, Maikel, primary, Primakov, Sergey, additional, Compter, Inge, additional, Woodruff, Henry C., additional, van Kuijk, Sander M. J., additional, Ramaekers, Bram L. T., additional, te Dorsthorst, Maarten, additional, Revenich, Elles G. M., additional, ter Laan, Mark, additional, Pegge, Sjoert A. H., additional, Meijer, Frederick J. A., additional, Beckervordersandforth, Jan, additional, Speel, Ernst Jan, additional, Kusters, Benno, additional, de Leng, Wendy W. J., additional, Anten, Monique M., additional, Broen, Martijn P. G., additional, Ackermans, Linda, additional, Schijns, Olaf E. M. G., additional, Teernstra, Onno, additional, Hovinga, Koos, additional, Vooijs, Marc A., additional, Tjan-Heijnen, Vivianne C. G., additional, Eekers, Danielle B. P., additional, Postma, Alida A., additional, Lambin, Philippe, additional, and Hoeben, Ann, additional
- Published
- 2021
- Full Text
- View/download PDF
7. Cell-Free HPV-DNA as a Biomarker for Oropharyngeal Squamous Cell Carcinoma-A Step Towards Personalized Medicine?
- Author
-
Steffen Wagner, Jens Peter Klussmann, Anne Adams, Ernst-Jan M. Speel, Nora Wuerdemann, Simon A. Joosse, Rishabh Jain, Pathologie, and RS: GROW - R2 - Basic and Translational Cancer Biology
- Subjects
0301 basic medicine ,Oncology ,Cancer Research ,medicine.medical_specialty ,Cochrane Library ,lcsh:RC254-282 ,Article ,DISEASE ,cell-free DNA ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,SURVEILLANCE ,medicine ,Liquid biopsy ,human papillomavirus ,RECURRENCE ,HUMAN-PAPILLOMAVIRUS DNA ,liquid biopsy ,PLASMA ,business.industry ,Area under the curve ,virus diseases ,Cancer ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,CANCER ,female genital diseases and pregnancy complications ,CIRCULATING TUMOR-CELLS ,meta-analysis ,INSIGHTS ,030104 developmental biology ,030220 oncology & carcinogenesis ,Meta-analysis ,Diagnostic odds ratio ,oropharyngeal squamous cell carcinoma ,Biomarker (medicine) ,biomarker ,Personalized medicine ,business ,LOCALLY ADVANCED HEAD ,LIQUID BIOPSIES - Abstract
Global incidences of oropharyngeal squamous cell carcinoma (OPSCC) are rising due to an association with high-risk human papillomavirus (HPV). Although there is an improved overall survival of HPV-related OPSCC, up to 25% of the patients develop recurrent or distant metastatic disease with a fatal outcomes. Biomarkers to monitor this disease are not established. This meta-analysis reviews the role of cell-free HPV DNA in liquid biopsy (LB) as a biomarker for HPV-related OPSCC. Pubmed, Livivo, and Cochrane Library databases were searched from inception to August, 2020. All studies were analyzed by Meta-DiSc 1.4 and Stata 16.0 statistical software. In total, 16 studies were considered for systematic review, whereas 11 studies met inclusion criteria for meta-analysis, respectively. Pooled sensitivity of cfHPV-DNA at first diagnosis and during follow-up was 0.81 (95% CI, 0.78&ndash, 0.84) and 0.73 (95% CI, 0.57&ndash, 0.86), while pooled specificity was 0.98 (95% CI, 0.96&ndash, 0.99) and 1 (95% CI, 0.99&ndash, 1). The diagnostic odds ratio (DOR) at first diagnosis was 200.60 (95% CI, 93.31&ndash, 431.22) and 300.31 (95% CI, 60.94&ndash, 1479.88) during follow-up. The area under the curve (AUC) of summary receiver operating characteristic (SROC) was 0.99 at first diagnosis and 1.00 during follow-up, respectively. In conclusion, cfHPV-DNA presents a potential biomarker with high specificity in patients with HPV-related OPSCC.
- Published
- 2020
8. Upfront Surgery vs. Primary Chemoradiation in an Unselected, Bicentric Patient Cohort with Oropharyngeal Squamous Cell Carcinoma—A Matched-Pair Analysis
- Author
-
Philipp Wolber, Ernst-Jan M. Speel, Malte Suchan, Christine Langer, Nora Wuerdemann, Claus Wittekindt, Shachi Jenny Sharma, Philipp Heinrich Zimmermann, Hans Edmund Eckel, Steffen Wagner, Jens Peter Klussmann, Bernd Kremer, Marijn Stuut, Kathrin Möllenhoff, Fabian Kämmerer, RS: GROW - R2 - Basic and Translational Cancer Biology, KNO, MUMC+: MA AIOS Keel Neus Oorheelkunde (9), and Pathologie
- Subjects
Cancer Research ,medicine.medical_specialty ,Matched Pair Analysis ,Complete data ,primary chemoradiation ,matched-pair analysis ,survival ,Article ,Therapeutic approach ,medicine ,Hazard model ,Oropharyngeal squamous cell carcinoma ,RC254-282 ,DEFINITIVE RADIOTHERAPY ,upfront surgery ,therapy ,business.industry ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Cancer ,medicine.disease ,CANCER-SURGERY ,Surgery ,Oncology ,Cohort ,oropharyngeal squamous cell carcinoma ,business ,Chemoradiotherapy - Abstract
Simple Summary:& nbsp;Oropharyngeal squamous cell carcinoma (OPSCC) is a common malignancy of the upper aerodigestive tract with rising incidence. While surgical and non-surgical approaches are applied in curative treatment, none of these has proven superior to date. In this study, we investigated overall survival in an unselected, bicentric cohort of patients with OPSCC and compared upfront surgery vs. primary chemoradiation treatments. A matched-pair analysis was performed to exclude confounding factors and reduce bias. Our results suggest that regardless of the treatment modality chosen, overall survival rates are comparable in both cohorts. As a consequence, future studies on functional outcome of patients with OPSCC are mandatory to identify the treatment modality most likely resulting in improved quality of life in patients with OPSCC.The two pillars of therapy for oropharyngeal squamous cell carcinoma (OPSCC) are upfront surgery and primary chemoradiotherapy. Substantial regional preferences exist with regard to the selection of treatment. Despite new therapeutic approaches, patient survival remains poor, with an approximate overall survival (OS) rate of 50% at five years. This study was conducted to investigate a potential survival benefit depending on the treatment modality in OPSCC patients. We retrospectively collected data of 853 patients with histologically confirmed OPSCC from the Giessen and Maastricht cancer databases. To identify risk factors affecting survival, a Cox-proportional hazard model was applied to 442 patients with complete data sets. Based on this cohort a matched-pair analysis with 158 patients was performed to compare OS rates of patients treated either with upfront surgery or primary chemoradiation. For the collective cohort, patients treated with upfront surgery had significantly improved OS rates compared to patients treated with primary chemoradiation. In the matched-pair analysis adjusted for patients' T-, N- and HPV-status as well as risk profile, we observed that both treatment approaches offered equivalent OS rates. Our study emphasizes that treatment recommendations should be made whenever possible on the basis of side-effect profiles caused by the therapeutic approach used. To draw further conclusions, results of the ongoing "best of" (NCT2984410) study are eagerly awaited, investigating the functional outcome after treatment of OPSCC patients.
- Published
- 2021
9. Cell-Free HPV-DNA as a Biomarker for Oropharyngeal Squamous Cell Carcinoma—A Step Towards Personalized Medicine?
- Author
-
Wuerdemann, Nora, primary, Jain, Rishabh, additional, Adams, Anne, additional, Speel, Ernst-Jan M., additional, Wagner, Steffen, additional, Joosse, Simon A., additional, and Klussmann, Jens P., additional
- Published
- 2020
- Full Text
- View/download PDF
10. Orthopedia Homeobox (OTP) in Pulmonary Neuroendocrine Tumors
- Author
-
Ernst-Jan M. Speel, Anne-Marie C. Dingemans, Jules L. Derks, and Laura Moonen
- Subjects
0301 basic medicine ,Oncology ,EXPRESSION ,Cancer Research ,medicine.medical_specialty ,INSULINOMA-ASSOCIATED PROTEIN-1 ,GENES ,Carcinoid tumors ,review ,Disease ,Neuroendocrine tumors ,Stem cell marker ,lcsh:RC254-282 ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Stage (cooking) ,SPECIFICATION ,HYPERPLASIA ,CARCINOID-TUMORS ,business.industry ,Cancer ,Hyperplasia ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,CELL LINEAGES ,CANCER ,digestive system diseases ,orthopedia homeobox ,030104 developmental biology ,Lymphatic system ,DIFFERENTIATION ,classification ,030220 oncology & carcinogenesis ,EXPERIENCE ,prognosis ,business ,pulmonary carcinoid ,neuroendocrine tumor - Abstract
Generally, patients with stage I-IIIa (TNM) pulmonary carcinoid disease have a favourable prognosis after curative resection. Yet, distant recurrence of disease after curative surgery occurs in approximately 1−6% of patients with typical carcinoid and 14−29% in patients with atypical carcinoid disease, respectively. Known predictors of distant recurrence of disease are atypical carcinoid, lymphatic involvement, and incomplete resection status. However, none of them can be reliably used, alone or in combination, to exclude patients from long-term follow-up (advised 15 years). By genomic profiling, Orthopedia homeobox (OTP) has been identified as a promising prognostic marker for pulmonary carcinoid with a favourable prognosis and low risk of distant disease recurrence. Moreover, OTP is a highly specific marker for carcinoids of pulmonary origin and recent genome wide analysis has identified OTP as a crucial predictor of aggressive tumor behaviour. OTP in combination with CD44, a stem cell marker and cell-surface protein, enables the identification of patients with surgical resected carcinoid disease that could potentially be excluded from long-term follow-up. In future clinical practice OTP may enable clinicians to reduce the diagnostic burden and related distress and reduce costs of long-term radiological assessments in patients with a pulmonary carcinoid. This review addresses the current clinical value of OTP and the possible molecular mechanisms regulating OTP expression and function in pulmonary carcinoids.
- Published
- 2019
11. The Antiviral Agent Cidofovir Induces DNA Damage and Mitotic Catastrophe in HPV-Positive and -Negative Head and Neck Squamous Cell Carcinomas In Vitro
- Author
-
Ernst-Jan M. Speel, Imke Demers, Bernd Kremer, Dion Legemaate, Wisse Evert Haakma, Robin Jacobs, Femke Verhees, Mat Rousch, Promovendi ODB, KNO, MUMC+: MA AIOS Keel Neus Oorheelkunde (9), RS: GROW - R3 - Innovative Cancer Diagnostics & Therapy, RS: GROW - R2 - Basic and Translational Cancer Biology, Pathologie, MUMC+: Oncologie Centrum (3), MUMC+: MA Keel Neus Oorheelkunde (3), and MUMC+: MA Keel Neus Oorheelkunde (9)
- Subjects
0301 basic medicine ,Cancer Research ,DNA damage ,DNA repair ,viruses ,animal diseases ,cyclin B1 ,Cell ,LINES ,lcsh:RC254-282 ,Article ,03 medical and health sciences ,0302 clinical medicine ,medicine ,ACYCLIC NUCLEOSIDE PHOSPHONATES ,RADIOSENSITIVITY ,human papillomavirus ,Mitosis ,Mitotic catastrophe ,Aurora Kinase A ,REPAIR ,double-stranded DNA breaks ,Cell growth ,Chemistry ,HUMAN-PAPILLOMAVIRUS ,DEATH ,INHIBITOR ,virus diseases ,cell line ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Head and neck squamous-cell carcinoma ,APOPTOSIS ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,Apoptosis ,030220 oncology & carcinogenesis ,Cancer research ,SURVIVAL ,GROWTH ,head and neck cancer - Abstract
Cidofovir (CDV) is an antiviral agent with antiproliferative properties. The aim of our study was to investigate the efficacy of CDV in HPV-positive and -negative head and neck squamous cell carcinoma (HNSCC) cell lines and whether it is caused by a difference in response to DNA damage. Upon CDV treatment of HNSCC and normal oral keratinocyte cell lines, we carried out MTT analysis (cell viability), flow cytometry (cell cycle analysis), (immuno) fluorescence and western blotting (DNA double strand breaks, DNA damage response, apoptosis and mitotic catastrophe). The growth of the cell lines was inhibited by CDV treatment and resulted in &gamma, H2AX accumulation and upregulation of DNA repair proteins. CDV did not activate apoptosis but induced S- and G2/M phase arrest. Phospho-Aurora Kinase immunostaining showed a decrease in the amount of mitoses but an increase in aberrant mitoses suggesting mitotic catastrophe. In conclusion, CDV inhibits cell growth in HPV-positive and -negative HNSCC cell lines and was more profound in the HPV-positive cell lines. CDV treated cells show accumulation of DNA DSBs and DNA damage response activation, but apoptosis does not seem to occur. Rather our data indicate the occurrence of mitotic catastrophe.
- Published
- 2019
12. The Antiviral Agent Cidofovir Induces DNA Damage and Mitotic Catastrophe in HPV-Positive and -Negative Head and Neck Squamous Cell Carcinomas In Vitro
- Author
-
Verhees, Femke, primary, Legemaate, Dion, additional, Demers, Imke, additional, Jacobs, Robin, additional, Haakma, Wisse Evert, additional, Rousch, Mat, additional, Kremer, Bernd, additional, and Speel, Ernst Jan, additional
- Published
- 2019
- Full Text
- View/download PDF
13. Orthopedia Homeobox (OTP) in Pulmonary Neuroendocrine Tumors: The Diagnostic Value and Possible Molecular Interactions.
- Author
-
Moonen, Laura, Derks, Jules, Dingemans, Anne-Marie, and Speel, Ernst-Jan
- Subjects
DISEASE relapse ,CELL receptors ,GENE expression ,GENETIC techniques ,LUNG tumors ,NEUROENDOCRINE tumors ,TUMOR markers ,DNA-binding proteins ,GENE expression profiling - Abstract
Generally, patients with stage I-IIIa (TNM) pulmonary carcinoid disease have a favourable prognosis after curative resection. Yet, distant recurrence of disease after curative surgery occurs in approximately 1–6% of patients with typical carcinoid and 14–29% in patients with atypical carcinoid disease, respectively. Known predictors of distant recurrence of disease are atypical carcinoid, lymphatic involvement, and incomplete resection status. However, none of them can be reliably used, alone or in combination, to exclude patients from long-term follow-up (advised 15 years). By genomic profiling, Orthopedia homeobox (OTP) has been identified as a promising prognostic marker for pulmonary carcinoid with a favourable prognosis and low risk of distant disease recurrence. Moreover, OTP is a highly specific marker for carcinoids of pulmonary origin and recent genome wide analysis has identified OTP as a crucial predictor of aggressive tumor behaviour. OTP in combination with CD44, a stem cell marker and cell-surface protein, enables the identification of patients with surgical resected carcinoid disease that could potentially be excluded from long-term follow-up. In future clinical practice OTP may enable clinicians to reduce the diagnostic burden and related distress and reduce costs of long-term radiological assessments in patients with a pulmonary carcinoid. This review addresses the current clinical value of OTP and the possible molecular mechanisms regulating OTP expression and function in pulmonary carcinoids. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.