Search

Simple Summary: Leiomyosarcoma (LMS) is thought to be an immune cold tumor that generally does not respond to immune checkpoint inhibitors (ICIs). To date, there is no validated immune biomarker used in LMS patients. The tertiary lymphoid structure (TLS) is the only potential predictive biomarker, but rarely present in LMS. Our study is the first study to investigate the immune biomarker using comprehensive transcriptomic profiling solely focused on tumor immune microenvironment (TIME) in LMS. Our study identified a subset of LMS with an active ("hot") tumor immune microenvironment (TIME) that is consistently associated with several immune signatures validated in other cancers in the clinical setting. Our study supports the further development of TIME multi-gene immune signature predictive biomarker that can be embedded in the future prospective clinical trials to evaluate its clinical utility to select LMS patients for ICIs. Purpose: To investigate the immune biomarker in Leiomyosarcoma (LMS), which is rare and recognized as an immune cold cancer showing a poor response rate (<10%) to immune checkpoint inhibitors (ICIs). However, durable response and clinical benefit to ICIs has been observed in a few cases of LMS, including, but not only, LMS with tertiary lymphoid structure (TLS) structures. Patients and methods: We used comprehensive transcriptomic profiling and a deconvolution method extracted from RNA-sequencing gene expression data in two independent LMS cohorts, the International Cancer Genome Consortium (ICGC, N = 146) and The Cancer Genome Atlas (TCGA, N = 75), to explore tumor immune microenvironment (TIME) in LMS. Results: Unsupervised clustering analysis using the previously validated two methods, 90-gene signature and Cell-type Identification by Estimating Relative Subsets of RNA Transcripts (CIBERSORT), identified immune hot (I-H) and immune high (I-Hi) LMS, respectively, in the ICGC cohort. Similarly, immune active groups (T-H, T-Hi) were identified in the TCGA cohort using these two methods. These immune active ("hot") clusters were significantly associated, but not completely overlapping, with several validated immune signatures such as sarcoma immune class (SIC) classification and TLS score, T cell inflamed signature (TIS) score, immune infiltration score (IIS), and macrophage score (M1/M2), with more patients identified by our clustering as potentially immune hot. Conclusions: Comprehensive immune profiling revealed a subset of LMS with a distinct active ("hot") TIME, consistently associated with several validated immune signatures in other cancers. This suggests that the methodologies that we used in this study warrant further validation and development, which can potentially help refine our current immune biomarkers to select the right LMS patients for ICIs in clinical trials. [ABSTRACT FROM AUTHOR]