1. Long noncoding RNASchLAHsuppresses metastasis of hepatocellular carcinoma through interacting with fused in sarcoma
- Author
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Fangyu Zhao, Xin-Rong Yang, Dishui Gu, Wenxin Qin, Zhouhong Ge, Zhuoan Cheng, Hui Wang, Cun Wang, Ming Yao, Jia Fan, Xisong Huo, and Ning Wang
- Subjects
rac1 GTP-Binding Protein ,0301 basic medicine ,Cancer Research ,Pathology ,RHOA ,Carcinogenesis ,Metastasis ,0302 clinical medicine ,Cell Movement ,SchLAH ,Neoplasm Metastasis ,Mice, Inbred BALB C ,Gene knockdown ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,Effector ,Liver Neoplasms ,hepatocellular carcinoma ,Hep G2 Cells ,General Medicine ,Prognosis ,Long non-coding RNA ,Gene Expression Regulation, Neoplastic ,Oncology ,030220 oncology & carcinogenesis ,Hepatocellular carcinoma ,Original Article ,RNA Interference ,RNA, Long Noncoding ,Sarcoma ,Protein Binding ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,Blotting, Western ,Transplantation, Heterologous ,Mice, Nude ,Fused in sarcoma ,03 medical and health sciences ,Cell Line, Tumor ,medicine ,metastasis ,Animals ,Humans ,long noncoding RNA ,neoplasms ,RNA ,Original Articles ,medicine.disease ,digestive system diseases ,030104 developmental biology ,Cancer research ,biology.protein ,RNA-Binding Protein FUS ,rhoA GTP-Binding Protein - Abstract
Emerging evidence has indicated that deregulation of long non-coding RNAs (lncRNAs) can contribute to the progression and metastasis of human cancer, including hepatocellular carcinoma (HCC). However, the roles of most lncRNAs in HCC remain largely unknown. Here we found a long noncoding RNA termed SchLAH (seven chromosome locus associated with HCC; also called BC035072) was generally downregulated in HCC. Low expression of SchLAH was significantly correlated with shorter overall survival of HCC patients. In vitro and in vivo assays indicated that overexpression of SchLAH inhibited the migration and lung metastasis of HCC cells. Knockdown of SchLAH by siRNA pool promoted the migration of HCC cells. RNA pull-down and RNA immunoprecipitation assays demonstrated SchLAH physically interacted with fused in sarcoma (FUS). PCR array analysis showed that RhoA and Rac1 were the downstream effector molecules of SchLAH during HCC metastasis. Knockdown of FUS rescued the mRNA levels of RhoA and Rac1 that were repressed by SchLAH. These results suggest that SchLAH may suppress the metastasis of HCC cells by interacting with FUS, which indicates potential of SchLAH for the prognosis and treatment of HCC.
- Published
- 2017
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