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Your search keyword '"Wu, Anna M"' showing total 12 results
Start Over Author "Wu, Anna M" Journal cancer research
12 results on '"Wu, Anna M"'

1. Abstract A088: Radiopharmaceutical therapy for pancreatic cancer: Engineered anti-prostate stem cell antigen (PSCA) antibody demonstrates targeted imaging and antitumor effects in a syngeneic mouse model of pancreatic ductal adenocarcinoma (PDAC)

Author

Chen, Bao Ying, primary, Ahmed, Saad N., additional, Salazar, Felix, additional, Jaiswal, Sunidhi, additional, Farshbaf, Masoud, additional, Chean, Jennifer, additional, Sherman, Anakim, additional, Hong, Teresa, additional, Yazaki, Paul J., additional, Olafsen, Tove, additional, Zettlitz, Kirstin A., additional, and Wu, Anna M., additional

Published
2024
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2. An Effective Immuno-PET Imaging Method to Monitor CD8-Dependent Responses to Immunotherapy

Author

Tavaré, Richard, Escuin-Ordinas, Helena, Mok, Stephen, McCracken, Melissa N, Zettlitz, Kirstin A, Salazar, Felix B, Witte, Owen N, Ribas, Antoni, and Wu, Anna M

Subjects
Biomedical and Clinical Sciences, Oncology and Carcinogenesis, Immunology, Vaccine Related, Immunization, Biomedical Imaging, Cancer, Development of treatments and therapeutic interventions, 5.1 Pharmaceuticals, Animals, Antibodies, Bispecific, CD8 Antigens, CD8-Positive T-Lymphocytes, Colonic Neoplasms, Deferoxamine, Disease Models, Animal, Humans, Immunoconjugates, Immunotherapy, Adoptive, Lymphocytes, Tumor-Infiltrating, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Positron-Emission Tomography, Radioisotopes, Radiopharmaceuticals, Zirconium, Oncology & Carcinogenesis, Biochemistry and cell biology, Oncology and carcinogenesis
Abstract

The rapidly advancing field of cancer immunotherapy is currently limited by the scarcity of noninvasive and quantitative technologies capable of monitoring the presence and abundance of CD8(+) T cells and other immune cell subsets. In this study, we describe the generation of (89)Zr-desferrioxamine-labeled anti-CD8 cys-diabody ((89)Zr-malDFO-169 cDb) for noninvasive immuno-PET tracking of endogenous CD8(+) T cells. We demonstrate that anti-CD8 immuno-PET is a sensitive tool for detecting changes in systemic and tumor-infiltrating CD8 expression in preclinical syngeneic tumor immunotherapy models including antigen-specific adoptive T-cell transfer, agonistic antibody therapy (anti-CD137/4-1BB), and checkpoint blockade antibody therapy (anti-PD-L1). The ability of anti-CD8 immuno-PET to provide whole body information regarding therapy-induced alterations of this dynamic T-cell population provides new opportunities to evaluate antitumor immune responses of immunotherapies currently being evaluated in the clinic.

Published
2016

3. Abstract 5372: CD3 and CD8 targeting of ionizable lipid nanoparticles for in vivo mRNA delivery to T cells

Author

Robinson, Elise R., primary, Kare, Aris J., additional, Kheirolomoom, Azadeh, additional, Inayathullah, Mohammed, additional, Tumbale, Spencer K., additional, Wu, Bo, additional, Raie, Marina N., additional, Seo, Jai W., additional, Salazar, Felix B., additional, Paulmurugan, Ramasamy, additional, Wu, Anna M., additional, and Ferrara, Katherine W., additional

Published
2022
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6. Abstract 4300: Receptor occupancy and tumor penetration by antibodies, peptides, and antibody fragments: Molecular simulation of imaging assessment

Author

Orcutt, Kelly D., primary, Adams, Gregory P., additional, Wu, Anna M., additional, Silva, Matthew, additional, Hoppin, Jack, additional, Harwell, Catey, additional, Matsumura, Manabu, additional, Kotsuma, Masakatsu, additional, Freeman, Daniel, additional, Tse, Archie, additional, Greenberg, Jonathan, additional, Scott, Andrew, additional, and Beckman, Robert A., additional

Published
2014
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10. Optimizing Radiolabeled Engineered Anti-p185HER2 Antibody Fragments for In vivo Imaging

Author

Olafsen, Tove, primary, Kenanova, Vania E., additional, Sundaresan, Gobalakrishnan, additional, Anderson, Anne-Line, additional, Crow, Desiree, additional, Yazaki, Paul J., additional, Li, Lin, additional, Press, Michael F., additional, Gambhir, Sanjiv S., additional, Williams, Lawrence E., additional, Wong, Jeffrey Y.C., additional, Raubitschek, Andrew A., additional, Shively, John E., additional, and Wu, Anna M., additional

Published
2005
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11. Tailoring the Pharmacokinetics and Positron Emission Tomography Imaging Properties of Anti–Carcinoembryonic Antigen Single-Chain Fv-Fc Antibody Fragments

Author

Kenanova, Vania, primary, Olafsen, Tove, additional, Crow, Desiree M., additional, Sundaresan, Gobalakrishnan, additional, Subbarayan, Murugesan, additional, Carter, Nora H., additional, Ikle, David N., additional, Yazaki, Paul J., additional, Chatziioannou, Arion F., additional, Gambhir, Sanjiv S., additional, Williams, Lawrence E., additional, Shively, John E., additional, Colcher, David, additional, Raubitschek, Andrew A., additional, and Wu, Anna M., additional

Published
2005
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12. Optical bioluminescence and positron emission tomography imaging of a novel fusion reporter gene in tumor xenografts of living mice.

Author

Ray P, Wu AM, and Gambhir SS

Subjects
Amino Acid Sequence, Animals, Blotting, Western, Cell Line, Cnidaria enzymology, Gene Expression, Glioma diagnostic imaging, Glioma genetics, Glioma metabolism, Humans, Luciferases analysis, Luciferases biosynthesis, Luminescent Measurements, Mice, Molecular Sequence Data, Neoplasm Transplantation, Neoplasms, Experimental genetics, Neoplasms, Experimental metabolism, Neurons diagnostic imaging, Neurons metabolism, Neurons physiology, Optics and Photonics, Rats, Recombinant Fusion Proteins analysis, Recombinant Fusion Proteins biosynthesis, Recombinant Fusion Proteins genetics, Thymidine Kinase analysis, Thymidine Kinase biosynthesis, Tomography, Emission-Computed methods, Transfection, Transplantation, Heterologous, Tumor Cells, Cultured, Genes, Reporter, Luciferases genetics, Neoplasms, Experimental diagnostic imaging, Thymidine Kinase genetics
Abstract

Noninvasive imaging of reporter gene expression using various imaging modalitiesis playing an increasingly important role in defining molecular events in the field of cancer biology, cell biology, and gene therapy. In this study, a novel reporter vector was constructed encoding a fusion protein comprised of a mutant herpes simplex virus type 1 thymidine kinase (HSV1-sr39tk) (tk), a positron emission tomography (PET) reporter gene, and renilla luciferase (rl), a bioluminescence optical reporter gene joined by a 20 amino acid long spacer sequence. We validated the activity of the two enzymes encoded by the fusion protein (tk(20)rl) in cell culture. Then, tumors stably expressing the tk(20)rl fusion gene were imaged both by microPET and optically using a cooled charge coupled device camera in xenograft-bearing living mice. Using a single fusion reporter (PET/optical) gene should accelerate the validation of reporter gene approaches developed in cell culture for translation into preclinical and clinical models.

Published
2003

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