1. The production of acidic polysaccharides by 5-bromodeoxyuridine-treated B16 mouse melanoma cells.
- Author
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Sato C, Kreider JW, Banks J, Garlick S, and Davidson EA
- Subjects
- Animals, Cells, Cultured, Chondroitin biosynthesis, DNA metabolism, Depression, Chemical, Glucosamine metabolism, Glycopeptides biosynthesis, Heparitin Sulfate biosynthesis, Hyaluronic Acid biosynthesis, Mice, Neoplasms, Experimental metabolism, Proteoglycans biosynthesis, Sialic Acids, Thymidine pharmacology, Bromodeoxyuridine pharmacology, Melanoma metabolism, Polysaccharides biosynthesis
- Abstract
B16 melanoma cells were treated in culture with 5-bromo-2-deoxyuridine. The cell-associated and released proteoglycans and sialoglycopeptides were compared to those of control cultures treated with thymidine. The 5-bromo-2-deoxyuridine-treated cultures showed a marked reduction in the proportion of cell-associated proteoglycans and sialoglycopeptides, an increase in the synthesis of hyaluronic acid, the absence of high-molecular-weight chondroitin sulfate, and the presence of increased amounts of heparan sulfate in the media. In addition, the 5-bromo-2-deoxyuridine-treated cells had a higher DNA content and were larger than controls.
- Published
- 1975