1. The Genomic Relationship Between Primary Breast Carcinomas and Their Nodal Metastases
- Author
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Mohamed M. Desouki, Lori Shepherd, Joseph Geradts, Shaoxi Liao, Daniel P. Gaile, Norma J. Nowak, and Jeffrey M. Conroy
- Subjects
Cancer Research ,Candidate gene ,Pathology ,medicine.medical_specialty ,DNA Copy Number Variations ,Breast Neoplasms ,Biology ,Polymerase Chain Reaction ,Genome ,law.invention ,Metastasis ,Breast cancer ,law ,medicine ,Carcinoma ,Humans ,Genetic Association Studies ,Polymerase chain reaction ,Comparative Genomic Hybridization ,Principal Component Analysis ,Gene Expression Profiling ,Carcinoma, Ductal, Breast ,General Medicine ,medicine.disease ,Gene Expression Regulation, Neoplastic ,Oncology ,Lymphatic Metastasis ,Female ,NODAL ,Comparative genomic hybridization - Abstract
We screened the whole tumor genome to identify DNA copy number gains and losses that discriminate between primary breast carcinomas (MP) and their nodal metastases (ML). Six candidate genes were confirmed by quantitative PCR to have differentially distributed copy number changes. Three of the genes (ERRγ, DDX6, and TIAM1) were more commonly amplified in nodal metastases. Principal component analysis revealed that MP-ML pairs varied markedly in their genomic divergence. The latter was larger in PR-negative tumors. Nodal metastases may form early or late in the development of breast carcinomas and PR-negative tumors may metastasize earlier or are genomically less stable.
- Published
- 2011
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