1. Expression of Notch1 to -4 and their ligands in renal cell carcinoma: a tissue microarray study.
- Author
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Aparicio LM, Villaamil VM, Gallego GA, Caínzos IS, Campelo RG, Rubira LV, Estévez SV, Mateos LL, Perez JL, Vázquez MR, Calvo OF, and Bolós MV
- Subjects
- Adaptor Proteins, Signal Transducing, Adult, Aged, Aged, 80 and over, Biomarkers, Tumor biosynthesis, Calcium-Binding Proteins biosynthesis, Carcinoma, Renal Cell pathology, Cell Line, Tumor, Female, Humans, Immunohistochemistry, Intercellular Signaling Peptides and Proteins biosynthesis, Intracellular Signaling Peptides and Proteins, Kidney Neoplasms pathology, Male, Membrane Proteins biosynthesis, Middle Aged, Prognosis, Proteomics methods, Proto-Oncogene Proteins biosynthesis, Receptor, Notch1 biosynthesis, Receptor, Notch2 biosynthesis, Receptor, Notch3, Receptor, Notch4, Serrate-Jagged Proteins, Tissue Array Analysis, Carcinoma, Renal Cell metabolism, Kidney Neoplasms metabolism, Ligands, Receptors, Notch biosynthesis
- Abstract
Background: Mutations in signalling pathways essential for embryonic development often lead to tumourigenesis, as is also true for Notch. The aim of this study was to assess the relationship between Notch1 to -4 and their ligands with anatomopathological features of the patients with renal cell carcinoma (RCC)., Materials and Methods: This study investigated the pattern of protein expression in RCC specimens using tissue microarray technology. A total of 80 paraffin-embedded RCC samples were retrospectively analysed together with ACHN and A.704 cell lines., Results: Notch1 showed significant positive correlation with chromophobe RCC, no broken capsule, Furhman grade I and when the number of nodes involved was small [(N=1); p=0.039, 0.016, 0.037 and 0.001, respectively)]. Notch3 showed higher expression when the tumour was located in the right kidney (p=0.048)., Conclusion: Notch1 may be useful in the future as a biomarker for the differential diagnosis of different RCC histological subtypes. Notch1 to -3 may also have potential use as a strong prognostic factor.
- Published
- 2011