1. Branched-chain in situ hybridization for κ and λ light chains: A powerful ancillary technique for determining B-cell clonality in cytology samples
- Author
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David T. Ting, Miguel Rivera, Manoj Gandhi, Vikram Deshpande, Kshitij S. Arora, Lawrence R. Zukerberg, and Ivan Chebib
- Subjects
0301 basic medicine ,Cancer Research ,Pathology ,medicine.medical_specialty ,Population ,In situ hybridization ,Immunoglobulin light chain ,03 medical and health sciences ,0302 clinical medicine ,hemic and lymphatic diseases ,Cytology ,medicine ,B-cell lymphoma ,education ,B cell ,education.field_of_study ,biology ,medicine.disease ,Molecular biology ,Lymphoma ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,biology.protein ,Antibody - Abstract
BACKGROUND Current immunohistochemical and in situ hybridization (ISH) assays are generally inconclusive for clonality unless plasmacytic differentiation is present. This study examined a series of cytology specimens and explored the ability of a branched-chain RNA (bRNA) ISH assay for immunoglobulin κ constant (IGKC) and immunoglobulin λ constant (IGLC) to detect a clonal population of B lymphocytes. METHODS Pathology databases were used to identify fine-needle aspiration biopsies (n = 28) and exfoliative cytology samples (n = 20). Demographic, flow cytometry, and excision biopsy results were recorded. bRNA ISH was performed on the Leica Bond platform with the following probes: IGKC, IGLC, immunoglobulin λ-like polypeptide 5 (IGLL5), and a housekeeping gene (HKG). RESULTS The bRNA ISH assay was validated with 30 surgical biopsies. On bRNA ISH, a clonal B-cell population (light-chain ratio > 10:1) was detected in 22 of 28 cases with a final diagnosis of lymphoma. In 2 cases, a κ predominance was present, although the ratio was
- Published
- 2015