1. Sperm performance under hypoxic conditions in the intertidal fish Porichthys notatus
- Author
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Fitzpatrick, John L., Craig, Paul M., Bucking, Carol, Balshine, Sigal, Wood, Chris M., and McClelland, Grant B.
- Subjects
Toadfishes -- Physiological aspects -- Growth -- Observations ,Spermatozoa -- Motility ,Zoology and wildlife conservation ,Company growth ,Observations ,Physiological aspects ,Growth - Abstract
Hypoxia (low oxygen) exposure generally leads to decreased reproductive capacity, exhibited by reductions in testicular mass, reproductive hormones, and sperm swimming speed. However, in many fish species, reproduction occurs either periodically or exclusively under hypoxic conditions. In this study we assessed how hypoxia influences sperm performance in the plainfin midshipman (Porichthys notatus Girard, 1854), a species that lives in intertidal nests that become hypoxic during low tides. We exposed sperm from the same male to normoxic or hypoxic conditions and compared sperm characteristics and oxygen consumption between treatments. Sperm exposed to hypoxic water swam faster and consumed more oxygen than sperm swimming in normoxic conditions. Sperm swimming speed was positively related with oxygen consumption. For each male, the percentage of motile spermatozoa did not differ between treatments, suggesting that the same number of sperm were active but their performance was dependent on the dissolved oxygen content in the water. We discuss the implications of our results in the context of sperm competition and fertilization success under hypoxic conditions. Une exposition a l'hypoxie (faibles concentrations d'oxygene) mene generalement a une diminution de la capacite reproductive, ce qui se manifeste par une reduction de la masse testiculaire, des hormones reproductives et de la vitesse de nage des spermatozoides. Cependant, chez plusieurs especes de poissons, la reproduction a lieu periodiquement on meme exclusivement dans des conditions d'hypoxie. Dans notre etude, nous evaluons les influences de l'hypoxie sur la performance des spermatozoides chez le pilotin tachete (Porichthys notatus Girard, 1854), une espece qui vit dans des nids intertidaux qui deviennent hypoxiques a maree basse. Nous avons expose les spermatozoides d'un meme male a des conditions normoxiques on hypoxiques et avons compare les caracteristiques des spermatozoides et leur consommation d'oxygene dans les deux conditions experimentales. Les spermatozoides exposes a l'eau hypoxique nagent plus rapidement et consomment plus d'oxygene que les spermatozoides qui nagent en conditions normoxiques. Il y a une relation positive entre la vitesse de nage des spermatozoides et leur consommation d'oxygene. Chez un meme male, le pourcentage de spermatozoides mobiles ne varie pas d'un traitement a 1'autre, ce qui laisse croire qu'il y a le meme nombre de spermatozoides en activite, mais que leur performance depend de la concentration d'oxygene dissous dans l'eau. Nous discutons des implications de nos resultats dans le cadre de la competition spermatique et du succes de la fecondation dans des conditions hypoxiques. [Traduit par la Redaction], Introduction Environmental exposure to hypoxia (low oxygen) typically impairs reproductive functions in males. For example, in humans, rodents, and primates, males exposed to hypobaric hypoxia have disrupted testicular development, produce [...]
- Published
- 2009