1. Supplemental hydrogen sulfide in models of renal transplantation after cardiac death
- Author
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Smriti, Juriasingani, Vicky, Vo, Masoud, Akbari, Jaskiran, Grewal, Max, Zhang, Jifu, Jiang, Aaron, Haig, and Alp, Sener
- Subjects
Adenosine ,Swine ,Allopurinol ,Organ Preservation Solutions ,Kidney ,Glutathione ,Kidney Transplantation ,Rats ,Death ,Mice ,Raffinose ,Reperfusion Injury ,Animals ,Humans ,Insulin ,Surgery ,Hydrogen Sulfide - Abstract
The increasing use of kidneys from donations after cardiac death (DCD) for renal transplantation is hindered by negative outcomes owing to organ injury after prolonged warm and cold ischemia-reperfusion. Recently, hydrogen sulfide (HWe tested a novel 30-day in vivo syngeneic murine model of DCD renal transplantation, in which the donor kidney was clamped for 30 minutes and stored for 18 hours in cold University of Wisconsin (UW) solution or UW with 150 μM sodium hydrogen sulfide (UW + NaHS) before transplantation. We also tested a 7-day in vivo porcine model of DCD renal autotransplantation, in which the left kidney was clamped for 60 minutes and preserved for 24 hours using hypothermic perfusion with UW or UW + 150 μM NaHS before autotransplantation. We collected blood and urine samples periodically, and collected kidney samples at the end point for histopathology and quantitative reverse transcription polymerase chain reaction.Rats that received HOur findings support the potential use of H
- Published
- 2022