Objective: To evaluate different imaging protocols, especially with respect to radio frequency (RF) receiver coil location, for their suitability in providing least squares derived quantitative T2 values of ovarian follicular fluid for investigations of basic ovarian physiology. Methods: The ovaries of 10 women were imaged via magnetic resonance imaging (MRI) using externally positioned and intravaginally placed RF receiver coils. Half-Fourier acquisition with single-shot turbo spin-echo (HASTE), multiple-echo T2, Dixon, turbo spin-echo, and 3-dimensional (3D) fast imaging with steady-state precession (FISP) and time-reversed FISP (PSIF) sequences were used. Quantitative T2 nuclear spin relaxation rate information from the ovarian follicles between data acquired with the external and intravaginal coils were compared. Additionally, the amount of ovarian follicle and corpora lutea structural detail visible was qualitatively assessed. Results: The T2 computations indicated that there was no difference in the follicular fluid T2 values or in the heterogeneity (spatial variance) of the T2 values between data acquired with the external RF coil and data acquired with the intravaginal RF coil. The best sequences for the visualization of ovarian internal structure were the 3D PSIF sequences and the multiple-echo T2-weighted images, confirming our earlier imaging work on excised cow ovaries. Conclusion: It is best to use an externally placed RF coil for quantitative MRI study of ovarian physiology given the lack of difference in quantitative T2 information and the difficulty associated with imaging the ovaries using an intravaginal RF probe. [ABSTRACT FROM AUTHOR]