1. ELISA tests for dapsone and pyrimethamine and their application in a malaria chemoprophylaxis programme
- Author
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Greenwood, BM, Greenwood, AM, Bradley, AK, Shenton, FC, Smith, AW, Snow, RW, Williams, K, Eggelte, TA, Huikeshoven, H, and de Wit, M
- Abstract
Enzyme-linked immunosorbent asays (ELISAs) are described for determining levels of dapsone and pyrimethamine in urine. Both assays have a sensitivity of about 20 mug/l and are reproducible, but each produces some false positives. The problem of false positive reactions was partially obviated by requiring positive results in both assays. In a pilot study involving 50 children aged 3 months to 4 years who were given a single dose of Maloprim (pyrimethamine + dapsone), 75% were positive for dapsone 7 days after administration of the drug, while 25% were still positive 15 days after its administration. The corresponding proportions for pyrimethamine were 73% and 30%, respectively. Comparison of the results obtained in a larger chemoprophylaxis trial with those from the pilot study indicated that the assays described could be used to investigate whether antimalarials had been taken.
- Published
- 1986