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Abstract We studied the direct and stromal cell-mediated effects of bone marrow Thy 1,2+ cells on the growth of granulocyte-macrophage and erythroid colonies from the bone marrow of CBA/CaLac mice with experimental neuroses (conflict situation and paradoxical sleep deprivation). Proliferation and differentiation of hemopoietic precursors during neuroses are controlled by regulatory T cells. In conflict situation Thy 1,2+ cells stimulate the growth of hemopoietic precursors, which is associated with their direct effect and interaction with adherent cells of the hemopoiesis-inducing microenvironment. The interaction of Thy 1,2+ cells with adherent bone marrow cells during paradoxical sleep deprivation stimulates the formation of only granulocyte-macrophage colonies. [ABSTRACT FROM AUTHOR]

Abstract We studied the mechanisms of erythropoiesis regulation in a conflict situation and during paradoxical sleep deprivation. Plastic reconstruction of the erythroid hemopoietic stem during experimental neuroses is regulated by a complex multicomponent and multilevel system. This system consists of central adrenergic structures, sympathetic part of the autonomic nervous system, and α- and β-adrenoceptors on erythroid precursors and cells of the hemopoietic microenvironment. Erythroid cells receive instructive information from the central nervous system via adrenoceptors on cells of the hemopoiesis-inducing microenvironment and erythroid precursors. Hyperplasia (conflict situation) and suppression of erythropoiesis (paradoxical sleep deprivation) are associated with specificity of central adrenergic regulatory mechanisms. [ABSTRACT FROM AUTHOR]

Abstract We studied changes in the erythroid hemopoietic stem in CBA/CaLac mice with experimental neuroses demonstrating good and poor learning capacities (conflict situation and paradoxical sleep deprivation followed by training in a 3-arm T-maze). The animals with different learning capacities exhibited pronounced hyperplasia of the erythroid hemopoietic stem in response to neurosis. Activation of the erythron in good learners was related to acceleration of division and maturation of erythroid precursors and enhanced formation of cell complexes containing central macrophage. In poor learners hyperplasia of the erythroid hemopoietic stem under conditions of conflict situation manifested in activation of proliferation and differentiation (against the background of decreased count of erythroid and mixed complexes in the bone marrow), while after paradoxical sleep deprivation followed by T-maze training this hyperplasia was associated with increased formation of additional hemopoietic islets (against the background of desynchronization of division and maturation of erythroid precursor cells). [ABSTRACT FROM AUTHOR]

We studied the effect of Kropanol on local regulatory mechanisms of hemopoiesis during paradoxical sleep deprivation. The regulatory effect of Kropanol on granulomonocytopoiesis was determined by increased binding capacity of stromal cells in relation to granulocyte-macrophage precursors and increased formation of mixed hemopoietic islets leading to accelerated maturation of granulocyte-macrophage precursors and hyperplasia of the granulomonocytic hemopoietic stem. Stimulation of erythropoiesis with Kropanol was associated with increased formation of erythroid hemopoietic islets, accelerated differentiation of erythroid precursors, and high content of erythroid cells in the bone marrow. Kropanol increased proliferative activity of erythroid precursors. Stimulation of these processes depended on enhanced production of short-distance humoral regulators of erythro- and granulomonocytopoiesis. [ABSTRACT FROM AUTHOR]

We studied the effects of various natural preparations, including extracts of Siberian ginseng, Rhodiola rosea, bergenia, and ginseng and pantohematogen on erythropoiesis under conditions of conflict situation. The test preparations were divided into 2 groups depending on their modulatory effect on intensified erythropoiesis under conditions of conflict situation. Some of them reduced (extracts of ginseng, bergenia, and Rhodiola rosea), while others increased the degree of hyperplasia in the erythropoietic stem (extract of Siberian ginseng and pantohematogen). The regulatory effect of preparations depended on activity of the corresponding neurotransmitter systems in the brain and local regulatory mechanisms of hemopoiesis. [ABSTRACT FROM AUTHOR]