1. Y-fluorescence of interphase nuclei, especially circulating lymphocytes.
- Author
-
Polani PE and Mutton DE
- Subjects
- Amniotic Fluid cytology, Female, Fetus, Fibroblasts, Humans, Klinefelter Syndrome genetics, Male, Microscopy, Fluorescence, Mouth Mucosa cytology, Mustard Compounds, Ovarian Diseases genetics, Pregnancy, Quinacrine, Sex Chromatin, Sex Chromosome Aberrations genetics, Sex Determination Analysis, Fluorescence, Lymphocytes cytology, Mitosis, Sex Chromosomes
- Abstract
Application of a fluorescence technique for the detection of the human Y chromosome in interphase nuclei indicates that the best results may be obtained from the study of lymphocytes in blood smears. The most satisfactory Y-fluorescence is obtained with the use of buffered quinacrine mustard dihydrochloride. The method can be complemented by other standard techniques to obtain a clear idea of the sex-chromosome complement of the individual. The application of this technique to clinical and chromosomal diagnosis and to the prenatal detection of the Y chromosome in amniotic cells seems at least as promising as has proved the earlier identification of the Barr body.
- Published
- 1971
- Full Text
- View/download PDF