1. Serum adipokine profile and fatty acid composition of adipose tissues are affected by conjugated linoleic acid and saturated fat diets in obese Zucker rats
- Author
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Rui Pinto, Susana V. Martins, Cristina M. Alfaia, Rui J.B. Bessa, José A. M. Prates, Matilde Castro, Paula A. Lopes, Susana P. Alves, P.O. Rodrigues, and NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
- Subjects
Male ,obesity ,interleukin 1beta ,principal component analysis ,Conjugated linoleic acid ,Saturated fat ,cholesterol blood level ,Medicine (miscellaneous) ,Adipose tissue ,Palm Oil ,low density lipoprotein cholesterol ,chemistry.chemical_compound ,monounsaturated fatty acid ,Obese Zucker rats ,trans-10,cis-12-conjugated linoleic acid ,insulin resistance ,rat ,animal ,Linoleic Acids, Conjugated ,Epididymis ,chemistry.chemical_classification ,palm oil ,C reactive protein ,tumor necrosis factor alpha ,Nutrition and Dietetics ,saturated fatty acid ,retroperitoneal fat ,Fatty Acids ,article ,organ size ,Organ Size ,plasminogen activator inhibitor 1 ,polyunsaturated fatty acid ,Zucker rat ,Lipids ,adipose tissue ,fat intake ,Cholesterol ,vegetable oil ,Adipose Tissue ,Liver ,Saturated fatty acid ,Body Composition ,Polyunsaturated fatty acid ,lipid storage ,sheep ,medicine.medical_specialty ,Intra-Abdominal Fat ,Linoleic acid ,Saturated diets ,animal experiment ,adipocytokine ,interleukin 6 ,lipid liver level ,lipid composition ,Biology ,chemistry ,leptin ,Ovis ,animal tissue ,histology ,body weight ,liver weight ,SDG 3 - Good Health and Well-being ,Adipokines ,Dietary Fats, Unsaturated ,lipid ,blood ,atherogenic diet ,Internal medicine ,Plasminogen Activator Inhibitor 1 ,medicine ,epididymis fat ,Animalia ,Animals ,Plant Oils ,controlled study ,Obesity ,intraabdominal fat ,nonhuman ,Sheep ,adiponectin ,Rattus ,trans 10,cis 12 conjugated linoleic acid ,animal model ,Fatty acid ,Dietary Fats ,Rats ,Rats, Zucker ,Endocrinology ,inflammation ,edible oil ,fatty acid ,metabolism - Abstract
Funding: The authors acknowledge Clinica Médica e Diagnóstico Dr Joaquim Chaves (Alge´s, Portugal) for the technical assistance regarding the serum lipids determination. The present study was supported by FCT POCTI/CVT/2002/44 750 and PTDC/CVT/2006/66 114 grants. S. V. M., P. A. L. and S. P. A. are the recipients of FCT individual fellowships SFRH/BD/2005/2256, SFRH/BPD/2005/23 931 and SFRH/ BD/2007/37 793, respectively. S. V. M., P. A. L. and C. M. A. performed the animal experiment, tissue sampling, laboratory work and prepared the manuscript. P. O. R. was responsible for Luminex xMAP analysis. S. P. A. and R. J. B. B. were responsible for fatty acid analysis. R. M. A. P. performed the biochemistry serum profile. S. V. M. and R. J. B. B. performed the statistical analysis. M. F. C. and J. A. M. P. were responsible for interpretation of the results, preparation of the manuscript and design of the study. Conjugated linoleic acid (CLA) has been reported as having body fat lowering properties and the ability to modulate the inflammatory system in several models. In the present study, the effects of CLA added to saturated fat diets, from vegetable and animal origins, on the serum adipokine profile of obese Zucker rats were assessed. In addition, the fatty acid composition of epididymal and retroperitoneal adipose tissues was determined and a principal component analysis (PCA) was used to assess possible relationships between fatty acids and serum metabolites. Atherogenic diets (2% cholesterol) were formulated with palm oil and ovine fat and supplemented or not with 1% of a mixture (1:1) of cis-9, trans-11 and trans-10, cis-12-CLA isomers. CLA-fed animals exhibited lower daily feed intake, final body and liver weights, and hepatic lipids content. Total and LDL-cholesterol levels were increased in CLA-supplemented groups. CLA also promoted higher adiponectin and lower plasminogen activator inhibitor-1 (PAI-1) serum concentrations. In contrast to palm oil diets, ovine fat increased insulin resistance and serum levels of leptin, TNF-α and IL-1β. Epididymal and retroperitoneal adipose tissues had similar deposition of individual fatty acids. The PCA analysis showed that the trans-10, cis-12-CLA isomer was highly associated with adiponectin and PAI-1 levels. Summing up, CLA added to vegetable saturated enriched diets, relative to those from animal origin, seems to improve the serum profile of adipokines and inflammatory markers in obese Zucker rats due to a more favourable fatty acid composition. © 2009 The Authors. publishersversion published
- Published
- 2009