1. Blood outgrowth endothelial cells from chronic myeloid leukaemia patients are BCR/ABL1 negative
- Author
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Alexander Schultze, Judith Dierlamm, Philippe Schafhausen, Carsten Bokemeyer, Walter Fiedler, Sonja Loges, Jasmin Otten, Tim H. Brümmendorf, and Svenja Otterstetter
- Subjects
Angiogenesis ,Genes, abl ,Biology ,Polymerase Chain Reaction ,Immunophenotyping ,Blood cell ,Leukemia, Myelogenous, Chronic, BCR-ABL Positive ,hemic and lymphatic diseases ,Biomarkers, Tumor ,medicine ,Humans ,Progenitor cell ,In Situ Hybridization, Fluorescence ,Neovascularization, Pathologic ,Endothelial Cells ,Hematology ,medicine.disease ,Endothelial stem cell ,Haematopoiesis ,medicine.anatomical_structure ,Immunology ,Neoplastic Stem Cells ,Cancer research ,Stem cell ,Chronic myelogenous leukemia - Abstract
The existence of adult haemangioblasts with dual haematopoietic and endothelial developmental potential was confirmed after detection of Ph(+) vascular endothelial cells in chronic myeloid leukaemia (CML) patients. Blood outgrowth endothelial cells (OECs) from CML patients were found not to harbour the Philadelphia translocation and were thus not clonally related to BRC/ABL1(+) hematopoietic progenitors, but comprised a distinct subfraction of endothelial cells. Remarkably, the frequency of CML-derived OECs was 9-fold higher as compared to healthy donors (n = 19 and n = 300, respectively; P < 0.0001) and these cells showed increased proliferative potential, possibly reflecting the mobilisation of OEC progenitors by pro-angiogenic cytokines.
- Published
- 2008
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