1. Adult T-cell leukaemia/lymphoma-like human T-cell leukaemia virus-1 replication in infective dermatitis
- Author
-
Gabet, As, Kazanji, M., Couppie, P., Clity, E., Pouliquen, Jf, Sainte-Marie, D., Aznar, C., Wattel, E., Virologie et pathogenèse virale (VPV), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Laboratoire de Rétrovirologie [Cayenne, Guyane française], Institut Pasteur de la Guyane, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Service de Dermatologie, Centre Hospitalier Andre Rosemon, Laboratoire Hospitalo-Universitaire de Parasitologie-Mycologie, Coordination Régionale de la lutte contre le Virus de L'Immunodéficience Humaine (COREVIH)-Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française]-Université des Antilles (UA), A.S.G. is supported by the Centre Le´on Be´rard. This work was supported by grants from the Ministe`re de l'E´ducation Nationale, de la Recherche et de la Technologie (Programme de Recherche Fondamentale en Microbiologie et Maladies Infectieuses et Parasitaires), from the Association pour la Recherche sur le Cancer, from the Fondation Contre la Leuce´mie, from the Re´gion Rhoˆne-Alpes (programme e´mergence), and from the comite´ de´partemental de la Saoˆne et Loire de la Ligue Nationale Contre le Cancer., Rollin, Bertrand, Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Polymerase Chain Reaction/methods ,T-Lymphocytes ,Blotting, Western ,Leukemia-Lymphoma ,T-Lymphocytes/*virology ,Dermatitis ,Acute ,Research Support ,Antibodies, Viral ,Virus Replication ,Viral/blood ,Antiviral Agents ,Polymerase Chain Reaction ,Antibodies ,Western/methods ,immune system diseases ,hemic and lymphatic diseases ,Parasitic Diseases/immunology/virology ,Parasitic Diseases ,Humans ,Leukemia-Lymphoma, Adult T-Cell ,Lamivudine/therapeutic use ,Non-U.S. Gov't ,OCIS 000.1430 ,Child ,[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,Antiviral Agents/therapeutic use ,Human T-lymphotropic virus 1 ,HTLV-I-Associated/*immunology/parasitology ,Dermatitis/*immunology/parasitology/*virology ,Blotting ,Viral Load ,T-Cell ,Clone Cells ,Lamivudine ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,Regression Analysis ,Female ,Cell Division ,Human T-lymphotropic virus 1/immunology/*physiology - Abstract
Adult T-cell leukaemia/lymphoma (ATLL) is a malignant T-cell proliferation that occurs in 3-5% of individuals infected with human T-cell leukaemia virus-1 (HTLV-1). HTLV-1 infection is also linked to the development of infective dermatitis (ID), an exudative dermatitis of children that has been proposed as a cofactor of ATLL. Here, HTLV-1 replication was investigated over time in a girl with ID and multiparasitic infestation including strongyloidiasis, a disease also known to predispose HTLV-1 carriers to ATLL. Quantitative polymerase chain reaction (PCR) revealed extremely high proviral loads. During the 2-year period of the present study, the proportion of circulating infected cells ranged between 12% and 36%. Quadruplicate linker-mediated PCR amplification of HTLV-1 flanking sequences identified a pattern of extensive and persistent oligoclonal expansion of infected lymphocytes. As viral loads, both the number and the degree of infected T-cell expansion were independent of treatment or clinical signs. However, the temporal fluctuation of proviral loads correlated significantly with the degree of infected T-cell expansion, but not with the overall number of detected clones. This pattern of HTLV-1 replication over time is very different from that observed in asymptomatic carriers and reminiscent of that observed in ATLL, a result consistent with the proposal of ID as an ATLL cofactor.
- Published
- 2003