1. Minimal residual disease status as a predictor of relapse after allogeneic bone marrow transplantation for children with acute lymphoblastic leukaemia
- Author
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Derwood Pamphilon, C. G. Jones, E. L. Harris, Victoria Grandage, Jeremy Hancock, Jacqueline M. Cornish, A. W. Rowbottom, A Oakhill, E Clarke, C. J. C. Knechtli, R. J. Garland, Nick Goulden, Alan Lankester, Ann Green, M N Potter, and Colin G. Steward
- Subjects
medicine.medical_specialty ,Neoplasm, Residual ,Adolescent ,medicine.medical_treatment ,Gastroenterology ,Polymerase Chain Reaction ,Recurrence ,hemic and lymphatic diseases ,Acute lymphocytic leukemia ,Internal medicine ,Medicine ,Humans ,Transplantation, Homologous ,Child ,Bone Marrow Transplantation ,business.industry ,Infant, Newborn ,Infant ,Hematology ,Gene rearrangement ,Immunotherapy ,Precursor Cell Lymphoblastic Leukemia-Lymphoma ,medicine.disease ,Minimal residual disease ,Surgery ,Transplantation ,surgical procedures, operative ,medicine.anatomical_structure ,El Niño ,Child, Preschool ,Toxicity ,Bone marrow ,business ,Oligonucleotide Probes - Abstract
We have analysed the behaviour of minimal residual disease (MRD) after allogeneic bone marrow transplantation (allo-BMT) in 71 children with acute lymphoblastic leukaemia (ALL). The method relied on PCR of IgH, TCRdelta and/or TCRgamma gene rearrangements followed by electrophoretic size resolution and allele-specific oligoprobing. Patients were similarly conditioned; 55 received marrow from unrelated donors and 16 from related donors. MRD was assessed at various time-points up to 24 months after BMT. Three children were not evaluable due to transplant-related mortality. MRD was detected in 28/32 patients (88%) who relapsed post-BMT; 16 were positive at all times and 12 were initially negative but became positive at a median of 3 months (range 1.5-11) prior to relapse. In contrast, only eight of 36 (22%) patients who remained in continuing complete remission (CCR) (median follow-up 43 months, range 20-94) showed MRD at any time after BMT (P
- Published
- 1998