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5 results on '"P, Verrelle"'

1. In vivo emergence of a highly metastatic tumour cell line from a rat rhabdomyosarcoma after treatment with an alkylating agent

Author

E. Antoine, V. Lascaux, C. Pauwels, M.-F. Poupon, and P Verrelle

Subjects
Cytotoxicity, Immunologic, Cancer Research, Pathology, medicine.medical_specialty, Lung Neoplasms, Cell, Antineoplastic Agents, Biology, Streptozocin, Cell Line, chemistry.chemical_compound, In vivo, Nickel, Chlorozotocin, Rhabdomyosarcoma, medicine, Animals, Neoplasm Metastasis, Lung, Rats, Inbred Strains, medicine.disease, Rats, medicine.anatomical_structure, Oncology, chemistry, Cell culture, Karyotyping, Toxicity, Female, Lymph, Research Article
Abstract

Rats bearing a transplanted nickel-induced rhabdomyosarcoma (RMS 9-4/0), treated with chlorozotocin (CZT), an alkylating agent, showed an amplified metastatic invasion of the lung (median of 165 lung tumour nodules, compared to 3 for untreated controls). A higher level of metastatic invasion (200 nodules) was reached spontaneously after the grafting of the S4T line, which was obtained by successive in vivo passages of RMS 9-4/0 cells in CZT treated rats. S4T tumour cells also invaded the liver and a considerable proportion of the lymph nodes. The NT4T line, obtained by successive in vivo passages in untreated rats, showed a lesser degree of enhancement of metastatic capacity (57 nodules). Both derived lines proved to be more aggressive than the parental, proliferated more rapidly, and were resistant to CZT toxicity. Only the non-treated lineage became more resistant to NK lysis. The S4T line lost its myogenic differentiation and was best described as a fibrohistiosarcoma, whereas NT4T did not. Chromosome analysis demonstrated a reduced range of chromosome number per cell in both lines. We conclude that both S4T and NT4T tumours became more metastatic than RMS 9-4/0 as the result of tumour progression through in vivo passages, and that in addition S4T acquired a spontaneously higher metastatic potential, similar to that which occurred in rats grafted with RMS 9-4/0 or NT4T tumours and treated by CZT. This suggests an inheritable mutation in the S4T line. Images Figure 3

Published
1988

2. In vivo emergence of a highly metastatic tumour cell line from a rat rhabdomyosarcoma after treatment with an alkylating agent

Author

Antoine, E, Pauwels, C, Verrelle, P, Lascaux, V, and Poupon, MF

Abstract

Rats bearing a transplanted nickel-induced rhabdomyosarcoma (RMS 9-4/0), treated with chlorozotocin (CZT), an alkylating agent, showed an amplified metastatic invasion of the lung (median of 165 lung tumour nodules, compared to 3 for untreated controls). A higher level of metastatic invasion (200 nodules) was reached spontaneously after the grafting of the S4T line, which was obtained by successive in vivo passages of RMS 9-4/0 cells in CZT treated rats. S4T tumour cells also invaded the liver and a considerable proportion of the lymph nodes. The NT4T line, obtained by successive in vivo passages in untreated rats, showed a lesser degree of enhancement of metastatic capacity (57 nodules). Both derived lines proved to be more aggressive than the parental, proliferated more rapidly, and were resistant to CZT toxicity. Only the non-treated lineage became more resistant to NK lysis. The S4T line lost its myogenic differentiation and was best described as a fibrohistiosarcoma, whereas NT4T did not. Chromosome analysis demonstrated a reduced range of chromosome number per cell in both lines. We conclude that both S4T and NT4T tumours became more metastatic than RMS 9-4/0 as the result of tumour progression through in vivo passages, and that in addition S4T acquired a spontaneously higher metastatic potential, similar to that which occurred in rats grafted with RMS 9-4/0 or NT4T tumours and treated by CZT. This suggests an inheritable mutation in the S4T line.

Published
1988
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3. Interleukin-6 gene amplification and shortened survival in glioblastoma patients.

Author

Tchirkov A, Khalil T, Chautard E, Mokhtari K, Véronèse L, Irthum B, Vago P, Kémény JL, and Verrelle P

Subjects
Glioblastoma mortality, Humans, In Situ Hybridization, Fluorescence, Gene Amplification, Glioblastoma genetics, Interleukin-6 genetics
Abstract

Interleukin-6 (IL-6) is known to promote tumour growth and survival. We evaluated IL-6 gene amplification in tumours from 53 glioma patients using fluorescence in situ hybridisation. Amplification events were detected only in glioblastomas (15 out of 36 cases), the most malignant tumours, and were significantly associated with decreased patient survival.

Published
2007
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4. Clinical implications of quantitative real-time RT-PCR analysis of hTERT gene expression in human gliomas.

Author

Tchirkov A, Rolhion C, Kémény JL, Irthum B, Puget S, Khalil T, Chinot O, Kwiatkowski F, Périssel B, Vago P, and Verrelle P

Subjects
Adult, DNA-Binding Proteins, Female, Humans, Male, Middle Aged, Prognosis, RNA, Messenger genetics, RNA, Messenger metabolism, Survival Analysis, Time Factors, Gene Expression Regulation, Neoplastic, Glioma enzymology, Glioma genetics, Reverse Transcriptase Polymerase Chain Reaction methods, Telomerase genetics
Abstract

The presence of telomerase activity in a glioma may be a predictor of its malignant potential. Activation of telomerase is regulated at the transcriptional level of the human telomerase reverse transcriptase (hTERT). Here, we evaluated whether the amount of hTERT mRNA provides a molecular marker of glioma malignancy that would have clinical utility. We used a real-time RT-PCR to assess the number of hTERT transcripts in primary tumour samples derived from 70 glioma patients. Results were standardised by quantifying the number of ABL transcripts as internal control and expressed as hTERT/ABL ratio. The percentage of patients with detectable hTERT mRNA markedly increased with enhanced malignancy: low-grade gliomas expressed hTERT in one out of 14 cases (7.1%), anaplastic gliomas in four out of 13 cases (30.8%) and glioblastoma multiforme (GBM) tumours in 30 out of 43 cases (69.8%). The mean hTERT/ABL ratio was significantly higher in GBMs than in non-GBMs. Subdividing hTERT/ABL ratios as low (< pr = 25%) and high (>25%), we found that the overall survival among hTERT-positive GBMs was significantly worse in high hTERT expressors than in low hTERT expressors (P=0.0082). We conclude that the amount of hTERT mRNA may represent a diagnostic and prognostic indicator for GBM patients.

Published
2003
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5. IL-6 gene amplification and expression in human glioblastomas.

Author

Tchirkov A, Rolhion C, Bertrand S, Doré JF, Dubost JJ, and Verrelle P

Subjects
DNA Primers, DNA, Neoplasm analysis, Glioblastoma pathology, Humans, Interleukin-6 genetics, Polymerase Chain Reaction, Tumor Cells, Cultured, Gene Amplification, Gene Expression Regulation, Neoplastic, Glioblastoma genetics, Interleukin-6 biosynthesis
Abstract

The aggressiveness of human gliomas appears to be correlated with the upregulation of interleukin 6 (IL-6) gene. Using quantitative PCR methods, we detected amplification and expression of the IL-6 gene in 5 of 5 primary glioblastoma samples and in 4 of 5 glioblastoma cell lines. This finding suggests that the amplification of IL-6 gene may be a common feature in glioblastomas and may contribute to the IL-6 over-expression., (Copyright 2001 Cancer Research Campaign.)

Published
2001
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