1. Circulating tumour cells as a biomarker for diagnosis and staging in pancreatic cancer
- Author
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Ankeny, JS, Court, CM, Hou, S, Li, Q, Song, M, Wu, D, Chen, JF, Lee, T, Lin, M, Sho, S, Rochefort, MM, Girgis, MD, Yao, J, Wainberg, ZA, Muthusamy, VR, Watson, RR, Donahue, TR, Hines, OJ, Reber, HA, Graeber, TG, Tseng, HR, and Tomlinson, JS
- Subjects
Biomedical and Clinical Sciences ,Clinical Sciences ,Oncology and Carcinogenesis ,Genetics ,Clinical Research ,Rare Diseases ,Pancreatic Cancer ,Digestive Diseases ,Cancer ,4.1 Discovery and preclinical testing of markers and technologies ,Detection ,screening and diagnosis ,4.2 Evaluation of markers and technologies ,Biomarkers ,Tumor ,Carcinoma ,Pancreatic Ductal ,Cohort Studies ,Humans ,Neoplasm Staging ,Neoplastic Cells ,Circulating ,Pancreatic Neoplasms ,Proto-Oncogene Proteins p21(ras) ,pancreatic cancer ,circulating tumour cells ,biomarker ,staging ,Public Health and Health Services ,Oncology & Carcinogenesis ,Oncology and carcinogenesis - Abstract
BackgroundCurrent diagnosis and staging of pancreatic ductal adenocarcinoma (PDAC) has important limitations and better biomarkers are needed to guide initial therapy. We investigated the performance of circulating tumour cells (CTCs) as an adjunctive biomarker at the time of disease presentation.MethodsVenous blood (VB) was collected prospectively from 100 consecutive, pre-treatment patients with PDAC. Utilising the microfluidic NanoVelcro CTC chip, samples were evaluated for the presence and number of CTCs. KRAS mutation analysis was used to compare the CTCs with primary tumour tissue. CTC enumeration data was then evaluated as a diagnostic and staging biomarker in the setting of PDAC.ResultsWe found 100% concordance for KRAS mutation subtype between primary tumour and CTCs in all five patients tested. Evaluation of CTCs as a diagnostic revealed the presence of CTCs in 54/72 patients with confirmed PDAC (sensitivity=75.0%, specificity=96.4%, area under the curve (AUROC)=0.867, 95% CI=0.798-0.935, and P
- Published
- 2016