1. Overexpression of the Axl tyrosine kinase receptor in cutaneous SCC-derived cell lines and tumours.
- Author
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Green, J., Ikram, M., Vyas, J., Patel, N., Proby, C. M., Ghali, L., Leigh, I. M., O'toole, E. A., and Storey, A.
- Subjects
SKIN cancer ,CANCER cells ,SQUAMOUS cell carcinoma ,PROTEIN-tyrosine kinases ,GENE expression ,POLYMERASE chain reaction ,PROTEIN metabolism ,RNA metabolism ,GENES ,IMMUNOHISTOCHEMISTRY ,PROTEINS ,RESEARCH funding ,SKIN tumors ,TRANSFERASES ,REVERSE transcriptase polymerase chain reaction ,OLIGONUCLEOTIDE arrays ,GENE expression profiling ,CANCER cell culture ,NEOPLASTIC cell transformation - Abstract
The molecular mechanisms that underlie the development of squamous cell skin cancers (SSC) are poorly understood. We have used oligonucleotide microarrays to compare the differences in cellular gene expression between a series of keratinocyte cell that mimic disease progression with the aim of identifying genes that may potentially contribute towards squamous cell carcinoma (SCC) progression in vivo, and in particular to identify markers that may serve as potential therapeutic targets for SCC treatment. Gene expression differences were corroborated by polymerase chain reaction and Western blotting. We identified Axl, a receptor tyrosine kinase with transforming potential that has also been shown to have a role in cell survival, adhesion and chemotaxis, was upregulated in vitro in SCC-derived cells compared to premalignant cells. Extending the investigation to tumour biopsies showed that the Axl protein was overexpressed in vivo in a series of SCCs. [ABSTRACT FROM AUTHOR]
- Published
- 2006
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