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Start Over Author "Jéssica de Souza Andrade" Journal brazilian journal of veterinary research and animal science
3 results on '"Jéssica de Souza Andrade"'

1. Prostaglandin E2 induces ovulation in prepubertal mice

Author

Jéssica de Souza Andrade, Juliana Pavan Zuliani, Jaswant Singh, Sulamita da Silva Setúbal, Renata Reis da Silva, Augusto Schneider, and Luiz Francisco Machado Pfeifer

Subjects
Ovulation, Prostaglandin, Receptor, Puberty, Animal culture, SF1-1100
Abstract

The objective of this study was to determine the ability of prostaglandin E2 (PGE2) to induce ovulation and expression of PGE2 receptor (EP2 and EP4) and COX genes (COX-1 and COX-2) in the ovary and pituitary of prepubertal mice. The positive control consisted of the application of 5 μg of gonadotropin-releasing hormone (GnRH, n = 29); the negative control applied 0.5 mL of phosphate buffered saline (PBS, n=31); the treatment tested the application of 250 μg of PGE2 (n = 29), making a total of 89 prepubertal mice (BALB/c). Mice were euthanized 14 to 15 h after treatments to detect ovulation and tissue collection. A Chi-square test was used to compare the proportion of animals ovulating. Gene expressions and number of ovulation were analyzed by one-way ANOVA and Tukey’s test was used to compare means among groups. A greater proportion of mice (P < 0.001) ovulated after receiving GnRH (89.7%, 26/29) compared to PGE2 group (58.6%, 17/29). However, the proportion was higher compared to those treated with PBS (0%, 0/31). Ep2 gene expression in the pituitary was > two-fold higher (P < 0.05) in the PGE2 group compared to the PBS and GnRH groups. Further, PGE2 stimulated Cox1 (2.7 fold, P < 0.05) while GnRH stimulated Cox2 expression (6.5 fold, P < 0.05) in the pituitary when compared to the PBS group. In conclusion, our results support the hypothesis that PGE2 can induce ovulation in prepubertal mice with a concomitant increase in Ep2 and Cox1 gene expression in the pituitary gland.

Published
2021

2. The role of prostaglandin F2α on ovulation and LH release in cows

Author

Natália Ávila de Castro, Carlos Eduardo Porciuncula Leonardi, Jaswant Singh, Augusto Schneider, Paulo Bayard Gonçalves, Fernando Caetano Oliveira, Camila Amaral D’Ávila, Rogério Ferreira, Bernardo Garziera Gasperin, Elizângela Mírian Moreira, Jéssica de Souza Andrade, and Luiz Francisco Machado Pfeifer

Subjects
Cow, Ovary, Pituitary, Prostaglandin, Reproduction, Animal culture, SF1-1100
Abstract

This study aimed to evaluate the role of prostaglandin F2α (PGF) on ovulation. In Experiment 1, cows were randomly allocated to two treatments to receive 150 μg of d-Cloprostenol (PGF Group, n = 12) or 2 mL of NaCl 0.9% (Control Group, n = 11) and CIDRs, were removed 4 days later. No cow ovulated in Control and PGF groups. In Experiment 2, cows were randomly separated into two experimental groups to receive 4 injections of 150 μg of d-Cloprostenol (n = 9) or 2 mL of NaCL 0.9% (n = 9). In this experiment, ovulation was not observed in any cows. In Experiment 3, ovariectomized cows receive three injections of 300μg of PGF analog (PGF Group, n = 5), 100μg of Lecirelin (GnRH Group, n = 5) or 2 mL of PBS (Control Group, n = 4). The LH concentration was higher (P 11.5 mm) were treated with Saline (Control Group, n = 6); Lecirelin (GnRH Group, n = 7) or Cloprostenol Sodium (PGF Group, n = 6). There was a significant increase in the vascular area of follicles from 0 to 24 h in GnRH and PGF treatments. In conclusion, PGF was not able to induce ovulation in cows with high or low plasma progesterone concentration. Additionally, PGF alone was not able to induce LH release and follicle luteinization, but increased follicular vascularization.

Published
2021

3. The role of prostaglandin F2α on ovulation and LH release in cows

Author

Jaswant Singh, Luiz Francisco Machado Pfeifer, Augusto Schneider, Rogério Ferreira, Fernando Caetano de Oliveira, N. A. Castro, Jéssica de Souza Andrade, C. E. P. Leonardi, Paulo Bayard Dias Gonçalves, Elizângela Mírian Moreira, Bernardo Garziera Gasperin, Camila Amaral D’Ávila, NATÁLIA ÁVILA DE CASTRO, Universidade Federal de Pelotas (UFPel), CAMILA AMARAL D'ÁVILA, Universidade Federal de Pelotas (UFPel), BERNARDO GARZIERA GASPERIN, Universidade Federal de Pelotas (UFPel), ELIZÂNGELA MÍRIAN MOREIRA, CPAF-RO, JÉSSICA DE SOUZA ANDRADE, Bionorte-Rede de Biodiversidade e Biotecnologia da Amazônia Legal, LUIZ FRANCISCO MACHADO PFEIFER, CPAF-RO., CARLOS EDUARDO PORCIUNCULA LEONARDI, University of Saskatchewan, Saskatoon – SK, Canada, JASWANT SINGH, University of Saskatchewan, Saskatoon – SK, Canada, AUGUSTO SCHNEIDER, Universidade Federal de Pelotas (UFPel), PAULO BAYARD GONÇALVES, Universidade Federal de Santa Maria (UFSM), FERNANDO CAETANO OLIVEIRA, Universidade Federal de Pelotas (UFel), and ROGÉRIO FERREIRA, Universidade Estadual de Santa Catarina

Subjects
Ovulation, endocrine system, Reprodução Animal, Cows, Prostaglandin, media_common.quotation_subject, Animal reproduction, Vaca, Cloprostenol, Andrology, Follicle, chemistry.chemical_compound, Follicular phase, Reprodução, media_common, lcsh:SF1-1100, General Veterinary, Chemistry, Reproduction, Cow, Ovary, F2a, Cloprostenol Sodium, Prostaglandina, Ovário, respiratory system, Ovulação, Pituitária, Pituitary, Prostaglandins, Ovariectomized rat, Plasma progesterone, lipids (amino acids, peptides, and proteins), lcsh:Animal culture, hormones, hormone substitutes, and hormone antagonists
Abstract

This study aimed to evaluate the role of prostaglandin F2a (PGF) on ovulation. In Experiment 1, cows were randomly allocated to two treatments to receive 150 ug of d-Cloprostenol (PGF Group, n = 12) or 2 mL of NaCl 0.9% (Control Group, n = 11) and CIDRs, were removed 4 days later. No cow ovulated in Control and PGF groups. In Experiment 2, cows were randomly separated into two experimental groups to receive 4 injections of 150 ug of d-Cloprostenol (n = 9) or 2 mL of NaCL 0.9% (n = 9). In this experiment, ovulation was not observed in any cows. In Experiment 3, ovariectomized cows receive three injections of 300ug of PGF analog (PGF Group, n = 5), 100ug of Lecirelin (GnRH Group, n = 5) or 2 mL of PBS (Control Group, n = 4). The LH concentration was higher (P 11.5 mm) were treated with Saline (Control Group, n = 6); Lecirelin (GnRH Group, n = 7) or Cloprostenol Sodium (PGF Group, n = 6). There was a significant increase in the vascular area of follicles from 0 to 24 h in GnRH and PGF treatments. In conclusion, PGF was not able to induce ovulation in cows with high or low plasma progesterone concentration. Additionally, PGF alone was not able to induce LH release and follicle luteinization, but increased follicular vascularization. O objetivo deste estudo foi avaliar o papel da prostaglandina F2a (PGF) na ovulação. No Experimento 1, as vacas foram alocadas aleatoriamente em dois tratamentos para receber 150 ug de d-Cloprostenol (Grupo PGF, n = 12) ou 2 mL de NaCl 0,9% (Grupo Controle, n = 11) e os CIDR, foram removidos 4 dias depois. Nenhuma vaca ovulou nos grupos Controle e PGF. No Experimento 2, as vacas foram separadas aleatoriamente em dois grupos experimentais para receber 4 injeções de 150 ug de d-Cloprostenol (n = 9) ou 2 mL de NaCL 0,9% (n = 9). Não foi observada ovulação em nenhum dos animais deste experimento. No Experimento 3, vacas ovariectomizadas receberam três injeções de 300ug de análogo de PGF (Grupo PGF, n = 5), 100ug de Lecirelina (Grupo GnRH, n = 5) ou 2 mL de PBS (Grupo Controle, n = 4). A concentração de LH foi maior (P 11,5 mm) foram tratadas com solução salina (Grupo Controle, n = 6), Lecirelina (Grupo GnRH, n = 7) ou Cloprostenol Sódico (Grupo PGF, n = 6). Houve um aumento significativo na área vascular dos folículos de 0 a 24h nos tratamentos com GnRH e PGF. Em conclusão, a PGF não foi capaz de induzir ovulação em vacas com alta ou baixa concentração plasmática de progesterona. Além disso, a PGF sozinha não foi capaz de induzir a liberação de LH e a luteinização do folículo, mas aumentou a vascularização folicular . Made available in DSpace on 2021-05-14T02:20:32Z (GMT). No. of bitstreams: 1 cpafro-18563.pdf: 1421435 bytes, checksum: dade7a90b48343362cf148d99ae6e08a (MD5) Previous issue date: 2021

Published
2021

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