1. Effects of combination treatment of dexamethasone and melatonin on brain injury in intracerebral hemorrhage model in rats
- Author
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Guo-biao Liang, Yixue Xue, Yun-hui Liu, and Zhi-qing Li
- Subjects
Male ,medicine.medical_specialty ,Time Factors ,Combination therapy ,Blotting, Western ,Interleukin-1beta ,Apoptosis ,Brain Edema ,Enzyme-Linked Immunosorbent Assay ,Blood–brain barrier ,Dexamethasone ,Permeability ,Statistics, Nonparametric ,Cerebral edema ,Proinflammatory cytokine ,Melatonin ,chemistry.chemical_compound ,Body Water ,Internal medicine ,medicine ,Animals ,Rats, Wistar ,Molecular Biology ,Cerebral Hemorrhage ,Peroxidase ,bcl-2-Associated X Protein ,Evans Blue ,Neurologic Examination ,Intracerebral hemorrhage ,Analysis of Variance ,Tumor Necrosis Factor-alpha ,business.industry ,General Neuroscience ,Brain ,medicine.disease ,Rats ,Oxidative Stress ,medicine.anatomical_structure ,Endocrinology ,Proto-Oncogene Proteins c-bcl-2 ,chemistry ,Blood-Brain Barrier ,Drug Therapy, Combination ,Neurology (clinical) ,business ,Developmental Biology ,medicine.drug - Abstract
The study was performed to investigate the effect of combination therapy with melatonin and dexamethasone (DEX) on brain injury in intracerebral hemorrhage (ICH) model in rats. Compared to the control group, combination therapy group with melatonin (10 mg/kg) and DEX (0.025 mg/kg) significantly reduced the degree of (1) brain edema, (2) the permeability of blood brain barrier (measured by Evans blue), (3) Oxidative stress (evaluated by malondialdehyde assay), (4) cytokines expression (tumor necrosis factor-alpha and interleukin-1beta), and (5) apoptosis (measured by Bax and Bcl-2 expression). In addition, we have also clearly demonstrated that the combination therapy significantly ameliorated neurologic scores. Taken together, our results clearly indicated for the first time that strategies targeting multiple proinflammatory pathways may be more effective than a single effector molecule for the treatment of ICH.
- Published
- 2009
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