1. Identification of genes that express in response to light exposure and express rhythmically in a circadian manner in the mouse suprachiasmatic nucleus
- Author
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Nanae Umeda, Shin-Ichi T. Inouye, Hirokazu Takahashi, Ryutaro Fukumura, Ryoko Araki, Masumi Abe, Mitsugu Sujino, Maki Nakahara, and Kazuya Mori
- Subjects
Male ,Light ,Period (gene) ,Receptors, Cytoplasmic and Nuclear ,Nerve Tissue Proteins ,Biology ,Mice ,Animals ,Circadian rhythm ,RNA, Messenger ,Cloning, Molecular ,Molecular Biology ,Gene ,In Situ Hybridization ,Genetics ,Regulation of gene expression ,Suprachiasmatic nucleus ,Reverse Transcriptase Polymerase Chain Reaction ,General Neuroscience ,Cell biology ,Circadian Rhythm ,Gene expression profiling ,Mice, Inbred C57BL ,Gene Expression Regulation ,Hypothalamus ,RNA ,Suprachiasmatic Nucleus ,Neurology (clinical) ,Signal transduction ,Developmental Biology ,Signal Transduction - Abstract
Most biological phenomena, including behavior and metabolic pathways, are governed by an internal clock system that is circadian (i.e., with a period of approximately 24 h) and is reset by light exposure from outside. In order to understand the molecular basis of the resetting mechanism of the clock, we attempted to isolate light-inducible transcripts in the suprachiasmatic nucleus, where the master clock resides, using a new gene expression profiling procedure. We identified 87 such transcripts, successfully cloned 60 of them and confirmed their light inducibility. Six of the 60 were already known to be light inducible and 17 are protein-coding transcripts registered in the public database that were not known to be light inducible. Induction is subjective night specific in most of the transcripts. Interestingly, 6 of the transcripts exhibit rhythmic expression in a circadian manner in the suprachiasmatic nucleus.
- Published
- 2005