1. Apatite content of collagen materials dose-dependently increases pre-osteoblastic cell deposition of a cement line-like matrix
- Author
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V. Dumas, Carole Fournier, Laurence Vico, Pierre Jurdic, Marie-Thérèse Linossier, Alain Guignandon, Aline Rattner, and Anthony Perrier
- Subjects
Histology ,Physiology ,Endocrinology, Diabetes and Metabolism ,Bone Matrix ,Biocompatible Materials ,Matrix (biology) ,Bone remodeling ,Substrate Specificity ,Mice ,Calcification, Physiologic ,Cell Movement ,Apatites ,Bone cell ,medicine ,Cell Adhesion ,Animals ,Osteopontin ,Cell adhesion ,Osteoblasts ,biology ,Chemistry ,Bone Cements ,Osteoblast ,Anatomy ,3T3 Cells ,Biomechanical Phenomena ,Fibronectins ,Fibronectin ,medicine.anatomical_structure ,Phenotype ,Solubility ,biology.protein ,Biophysics ,Collagen ,Oligopeptides ,Type I collagen - Abstract
Bone matrix, mainly composed of type I collagen and apatite, is constantly modified during the bone remodeling process, which exposes bone cells to various proportions of mineralized collagen within bone structural units. Collagen-mineralized substrates have been shown to increase osteoblast activities. We hypothesized that such effects may be explained by a rapid secretion of specific growth factors and/or deposition of specific matrix proteins. Using MC3T3-E1 seeded for 32 h on collagen substrates complexed with various apatite contents, we found that pre-osteoblasts in contact with mineralized collagen gave rise to a dose-dependent deposit of Vascular Endothelial Growth Factor-A (VEGF-A) and RGD-containing proteins such as osteopontin (OPN) and fibronectin (FN). This RGD-matrix deposition reinforced the cell adhesion to collagen-mineralized substrates. It was also observed that, on these substrates, this matrix was elaborated concomitantly to an increased cell migration, allowing a homogeneous coverage of the sample. This particular surface activation was probably done firstly to reinforce cell survival (VEGF-A) and adhesion (OPN, FN) and secondly to recruit and prepare surfaces for subsequent bone cell activity.
- Published
- 2010