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Start Over Author "Fufa, Dawo" Journal bmc veterinary research
2 results on '"Fufa, Dawo"'

1. Isolation, molecular characterization and sero-prevalence study of foot-and-mouth disease virus circulating in central Ethiopia

Author

Mishamo Sulayeman, Fufa Dawo, Bedaso Mammo, Daniel Gizaw, and Dereje Shegu

Subjects
Cattle, Central Ethiopia, FMD, Molecular characterization, Risk factors, Sero-prevalence, Veterinary medicine, SF600-1100
Abstract

Abstract Background Ethiopian livestock production and productivity is still very low due to widespread of diseases. Among the diseases, foot-and-mouth disease (FMD) is an extremely contagious and acute viral disease that causes significant economic problems in the country. A cross sectional study design was conducted from September 2015 to May 2016 to isolate and characterize FMD virus from outbreak cases; determine the sero-prevalence of antibodies against FMD virus (FMDV), and assess potential risk factors associated with sero-prevalence of the disease in selected areas of central Ethiopia. A multistage sampling technique was employed to select the study animals. Isolated viruses were characterized by antigen ELISA (IZLER, Brescia, Italy) and by genetic analysis of the sequence of the viral protein 1 (VP1). Sero-prevalence was determined using an ELISA for antibodies against non-structural proteins of FMDV based on the 3ABC proteins (ID Screen® FMD NSP Competition, ID-VET, Grabels, France). Risk factors for sero-prevalence of antibodies against FMD virus was investigated using logistic regression analysis. Result From outbreak investigation, 28.8% (n = 378) cattle showed signs and lesions suggestive of FMD and 34 samples were subjected to virus isolation. Twenty eight of these cultures exhibited cytopathic effect (CPE) and were serotyped as O, A and SAT 2 FMD viruses. One A and two SAT 2 isolates named A-ETH-19-2015, SAT 2-ETH-18-2015 and SAT 2-ETH-20-2015 were further characterized by phylogenetic analysis. The overall sero-prevalence of antibodies against non-structural proteins of FMDV was 24.2% (n = 574). Cattle herds with crossbreed cattle, with older cattle (> 2 years), and kept together with small ruminants had higher sero-prevalences of antibodies against FMDV (p

Published
2018
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2. First report of Toxoplasma gondii in camels (Camelus dromedarius) in Ethiopia: bioassay and seroepidemiological investigation

Author

Maria Vitale, Getachew Terefe, Hasen Awel Yunus, Tesfaye Sisay Tessema, Vincenzo Di Marco, Gebregergs Tesfamaryam, Fufa Dawo, and Endrias Zewdu Gebremedhin

Subjects
Male, Veterinary medicine, endocrine system, Camelus, Logistic regression, Mice, Risk Factors, Seroepidemiologic Studies, Direct agglutination test, Camel milk, medicine, Seroprevalence, Animals, Humans, Animal Husbandry, Seroepidemiology, Camel, General Veterinary, biology, T. gondii, Toxoplasma gondii, General Medicine, Odds ratio, DAT, biology.organism_classification, medicine.disease, veterinary(all), Toxoplasmosis, Toxoplasmosis, Animal, Herd, ELISA, Bioassay, Biological Assay, Female, Ethiopia, Toxoplasma, Research Article
Abstract

Background Toxoplasmosis is a major public health concern in many countries of the world. A cross-sectional and follow up experimental study designs were used for seroepidemiological and bioassay studies, respectively from November 2012 to April 2013. The objectives were to estimate the seroprevalence of T. gondii infection, to assess risk factors and to isolate the parasite from camels in the Fentale district, Ethiopia. A direct agglutination test (DAT) and indirect enzyme linked immunosorbent assay (ELISA) kits were used to test camel sera. Hearts and tongues (each 25 g) from 31 seropositive camels were bioassayed in mice. Associations between seroprevalence and potential risk factors (collected using a questionnaire survey) were analyzed using logistic regression. Results An overall T. gondii prevalence of 49.62% (220/455) by DAT and 40.49% (179/451) by indirect ELISA test were detected. Herd level seroprevalence of 96.77% (30/31) (95% CI: 83.30– 99.92) by DAT was recorded and it was significantly higher in areas where wild felids are present (P = 0.038). Multivariable logistic regression showed that the likelihood of acquiring T. gondii infection was significantly higher in camels in the Ilala pastoral association [PA] (82.26%) (Adjusted Odds ratio [aOR] = 10.8; P

Published
2014

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