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2 results on '"Kazuyoshi Gotoh"'

1. Enhancement of intestinal epithelial barrier function by Weissella confusa F213 and Lactobacillus rhamnosus FBB81 probiotic candidates in an in vitro model of hydrogen peroxide-induced inflammatory bowel disease

Author

Ni Nengah Dwi Fatmawati, Kazuyoshi Gotoh, I. Putu Bayu Mayura, Komang Ayu Nocianitri, Gede Ngurah Rsi Suwardana, Ni Luh Gede Yoni Komalasari, Yan Ramona, Masakiyo Sakaguchi, Osamu Matsushita, and I. Nengah Sujaya

Subjects
Probiotics, Weissella confusa F213, Lactobacillus rhamnosus FBB81, Trans epithelial resistance, ZO-1 protein, Inflammatory bowel diseases, Medicine, Biology (General), QH301-705.5, Science (General), Q1-390
Abstract

Abstract Objective Weissella confusa F213 (WCF213) and Lactobacillus rhamnosus FBB81 (LrFBB81) are two probiotic candidates isolated from humans in our previous study. Their functional activity on the mucosal barrier has not yet been adequately investigated. Therefore, the objective of this study was to investigate the effect of these strains on maintaining mucosal integrity in vitro. Caco-2 cell monolayers were pretreated with WCF213 and LrFBB81 before being exposed to hydrogen peroxide. The integrity of mucosal cells was evaluated by measuring the transepithelial resistance (TER), flux of FITC-labelled dextran, and ZO-1 protein distribution with the help of an immunofluorescence method. Results WCF213 was found to significantly maintain the TER better than the control hydrogen peroxide-treated cells (p

Published
2020
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2. Enhancement of intestinal epithelial barrier function by Weissella confusa F213 and Lactobacillus rhamnosus FBB81 probiotic candidates in an in vitro model of hydrogen peroxide-induced inflammatory bowel disease

Author

Yan Ramona, Masakiyo Sakaguchi, Ni Nengah Dwi Fatmawati, Komang Ayu Nocianitri, I Nengah Sujaya, Kazuyoshi Gotoh, Ni Luh Gede Yoni Komalasari, Gede Ngurah Rsi Suwardana, I Putu Bayu Mayura, and Osamu Matsushita

Subjects
0301 basic medicine, Trans epithelial resistance, 030106 microbiology, Cell, lcsh:Medicine, Inflammatory bowel diseases, Immunofluorescence, Weissella confusa F213, General Biochemistry, Genetics and Molecular Biology, Microbiology, law.invention, Tight Junctions, 03 medical and health sciences, chemistry.chemical_compound, Probiotic, Lactobacillus rhamnosus, ZO-1 protein, law, medicine, Distribution (pharmacology), Humans, Intestinal Mucosa, lcsh:Science (General), Hydrogen peroxide, lcsh:QH301-705.5, medicine.diagnostic_test, biology, Chemistry, Lacticaseibacillus rhamnosus, Probiotics, lcsh:R, General Medicine, Hydrogen Peroxide, biology.organism_classification, In vitro, Research Note, 030104 developmental biology, medicine.anatomical_structure, Dextran, lcsh:Biology (General), Weissella, Lactobacillus rhamnosus FBB81, Caco-2 Cells, lcsh:Q1-390
Abstract

Objective Weissella confusa F213 (WCF213) and Lactobacillus rhamnosus FBB81 (LrFBB81) are two probiotic candidates isolated from humans in our previous study. Their functional activity on the mucosal barrier has not yet been adequately investigated. Therefore, the objective of this study was to investigate the effect of these strains on maintaining mucosal integrity in vitro. Caco-2 cell monolayers were pretreated with WCF213 and LrFBB81 before being exposed to hydrogen peroxide. The integrity of mucosal cells was evaluated by measuring the transepithelial resistance (TER), flux of FITC-labelled dextran, and ZO-1 protein distribution with the help of an immunofluorescence method. Results WCF213 was found to significantly maintain the TER better than the control hydrogen peroxide-treated cells (p p p

Published
2020

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