1. Exercise-induced up-regulation of MMP-1 and IL-8 genes in endurance horses
- Author
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Stefano Capomaccio, Camillo Pieramati, Andrea Verini-Supplizi, Michela Felicetti, Katia Cappelli, and Maurizio Silvestrelli
- Subjects
Physiology ,Biology ,Matrix metalloproteinase ,Horse ,Peripheral blood mononuclear cell ,lcsh:Physiology ,exercise ,stress ,gene expression ,Fight-or-flight response ,Downregulation and upregulation ,Stress, Physiological ,Physiology (medical) ,Physical Conditioning, Animal ,Gene expression ,Animals ,Interleukin 8 ,Horses ,Gene ,lcsh:QP1-981 ,Interleukin-8 ,General Medicine ,Up-Regulation ,Immunology ,Physical Endurance ,Matrix Metalloproteinase 1 ,Research Article - Abstract
Background The stress response is a critical factor in the training of equine athletes; it is important for performance and for protection of the animal against physio-pathological disorders. In this study, the molecular mechanisms involved in the response to acute and strenuous exercise were investigated using peripheral blood mononuclear cells (PBMCs). Results Quantitative real-time PCR (qRT-PCR) was used to detect modifications in transcription levels of the genes for matrix metalloproteinase-1 (MMP-1) and interleukin 8 (IL-8), which were derived from previous genome-wide expression analysis. Significant up-regulation of these two genes was found in 10 horses that had completed a race of 90–120 km in a time-course experimental design. Conclusion These results suggest that MMP-1 and IL-8 are both involved in the exercise-induced stress response, and this represents a starting point from which to understand the adaptive responses to this phenomenon.
- Published
- 2009