1. Total RNA and genomic DNA of Lactobacillus gasseri OLL2809 induce interleukin-12 production in the mouse macrophage cell line J774.1 via toll-like receptors 7 and 9.
- Author
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Onishi K, Mochizuki J, Sato A, Goto A, and Sashihara T
- Subjects
- Animals, Cell Culture Techniques, Cell Line, Genome, Bacterial, Lactobacillus gasseri immunology, Macrophages cytology, Macrophages microbiology, Mice, Myeloid Differentiation Factor 88 metabolism, Phagocytosis, Signal Transduction, Up-Regulation, DNA, Bacterial immunology, Interleukin-12 metabolism, Lactobacillus gasseri genetics, Macrophages immunology, Membrane Glycoproteins metabolism, RNA, Bacterial immunology, Toll-Like Receptor 7 metabolism, Toll-Like Receptor 9 metabolism
- Abstract
Background: Lactobacillus gasseri OLL2809 can highly induce interleukin (IL)-12 production in immune cells. Even though beneficial properties of this strain for both humans and animals have been reported, the mechanism by which the bacteria induces the production of IL-12 in immune cells remains elusive. In this study, we investigated the mechanism of induction of IL-12 using a mouse macrophage cell line J774.1., Results: Inhibition of phagocytosis of L. gasseri OLL2809, and myeloid differentiation factor 88 and Toll-like receptors (TLRs) 7 and 9 signalling attenuated IL-12 production in J774.1 cells. Total RNA and genomic DNA of L. gasseri OLL2809, when transferred to the J774.1 cells, also induced IL-12 production. The difference in the IL-12-inducing activity of Lactobacilli is attributed to the susceptibility to phagocytosis, but not to a difference in the total RNA and genomic DNA of each strain., Conclusion: We concluded that total RNA and genomic DNA of phagocytosed L. gasseri OLL2809 induce IL-12 production in J774.1 cell via TLRs 7 and 9, and the high IL-12-inducing activity of L. gasseri OLL2809 is due to its greater susceptibility to phagocytosis.
- Published
- 2020
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