1. Preservation of sputum samples with cetylpyridinium chloride (CPC) for tuberculosis cultures and Xpert MTB/RIF in a low-income country
- Author
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Hellen Hiza, Basra Doulla, Mohamed Sasamalo, Jerry Hella, Lujeko Kamwela, Francis Mhimbira, Klaus Reither, Sebastien Gagneux, Levan Jugheli, and Lukas Fenner
- Subjects
Mycobacterium tuberculosis ,Tuberculosis ,Cetylpyridinium chloride ,CPC ,Sample transport ,Preservation ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Culture contamination with environmental bacteria is a major challenge in tuberculosis (TB) laboratories in hot and humid climate zones. We studied the effect of cetylpyridinium chloride (CPC) preservation on culture results and performance of Xpert MTB/RIF. Methods Consecutive sputum samples from microscopy smear-positive TB patients were collected. Two-hundred samples were equally split in two aliquots, one aliquot was treated with CPC and stored at ambient temperature for 7 days. The second aliquot was immediately processed. Samples were decontaminated for 20, 15 or 10 min, and subsequently cultured on Löwenstein-Jensen medium. Furthermore, 50 samples were stored for 7, 14 and 21 days, and 100 CPC-pretreated samples tested by Xpert MTB/RIF. Results CPC pretreated samples showed a higher culture yield compared to non-treated sputum samples across all decontamination times: 94% vs. 73% at 10 min (p = 0.01), 94% vs. 64% at 15 min (p = 0.004), and 90% vs. 52% at 20 min (p
- Published
- 2017
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