14 results on '"Ndongo, A."'
Search Results
2. Performance of case definitions and clinical predictors for influenza surveillance among patients followed in a rural cohort in Senegal
- Author
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Mamadou Aliou Barry, Florent Arinal, Cheikh Talla, Boris Gildas Hedible, Fatoumata Diene Sarr, Ibrahim Oumar Ba, Boly Diop, Ndongo Dia, and Muriel Vray
- Subjects
Performance ,Influenza ,Surveillance ,Senegal ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Influenza is a major cause of morbidity and mortality in Africa. However, a lack of epidemiological data remains for this pathology, and the performances of the influenza-like illness (ILI) case definitions used for sentinel surveillance have never been evaluated in Senegal. This study aimed to i) assess the performance of three different ILI case definitions, adopted by the WHO, USA-CDC (CDC) and European-CDC (ECDC) and ii) identify clinical factors associated with a positive diagnosis for Influenza in order to develop an algorithm fitted for the Senegalese context. Methods All 657 patients with a febrile pathological episode (FPE) between January 2013 and December 2016 were followed in a cohort study in two rural villages in Senegal, accounting for 1653 FPE observations with nasopharyngeal sampling and influenza virus screening by rRT-PCR. For each FPE, general characteristics and clinical signs presented by patients were collected. Sensitivity, Specificity, Positive Predictive Value (PPV) and Negative Predictive Value (NPV) for the three ILI case definitions were assessed using PCR result as the reference test. Associations between clinical signs and influenza infection were analyzed using logistic regression with generalized estimating equations. Sore throat, arthralgia or myalgia were missing for children under 5 years. Results WHO, CDC and ECDC case definitions had similar sensitivity (81.0%; 95%CI: 77.0–85.0) and NPV (91.0%; 95%CI: 89.0–93.1) while the WHO and CDC ILI case definitions had the highest specificity (52.0%; 95%CI: 49.1–54.5) and PPV (32.0%; 95%CI: 30.0–35.0). These performances varied by age groups. In children
- Published
- 2021
- Full Text
- View/download PDF
3. Epidemiological, clinical and genotypic features of human Metapneumovirus in patients with influenza-like illness in Senegal, 2012 to 2016
- Author
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Mamadou Malado Jallow, Amary Fall, Davy Kiori, Sara Sy, Déborah Goudiaby, Mamadou Aliou Barry, Malick Fall, Mbayame Ndiaye Niang, and Ndongo Dia
- Subjects
Human metapneumovirus ,Influenza-like illness ,Epidemiology ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Human metapneumovirus (HMPV) is a causal agent of acute respiratory infection, especially in primarily children. At the clinical level, HMPV is associated to several diseases including bronchitis, croup, pneumonia, bronchiolitis, reactive airway disease, chronic obstructive pulmonary disease and asthma exacerbations, specifically in children less than 5 years. Here, we carried out a retrospective pilot study, based on the processing of nasopharyngeal swabs, with a focus on the epidemiology and molecular characteristics of HMPV in Senegal. Methods This retrospective study was conducted from January 2012 to December 2016. Briefly, all outpatients presenting to healthcare sentinel sites were screened for surveillance enrollment and included if they met criteria for ILI. Naso-oropharyngeal swabs were collected from eligible participants. For viral respiratory pathogens detection, including HMPV, the Anyplex™ II RV16 Detection kit was used. A fragment of the hMPV F gene was targeted for sequencing. Results In total, 8209 patients with ILI were enrolled. Half of them (49.7%) were children under 5 years. Fever was the most common symptom followed by cough, and rhinitis. Three hundred eight patients were positive for HMPV (3.75%). 89 (28.9%) were detected as single infection. In co-infection cases, the most common co-infecting viruses were influenza, adenovirus and rhinovirus. HMPV detection rates in the different age groups varied significantly with the children under 5 years group accounting for 71.7% of positive patients. The temporal distribution pattern for HMPV infection showed a clear seasonal pattern with a higher activity during the rainy period (July–September). Phylogenetic analyses revealed that HMPV specimens circulating in Senegal were distributed into the two main genetic lineages, A and B. We also noted a co-circulation of both genetic lineages during the whole study period except in 2014. Conclusion In summary, the present study characterized the recent prevalence, seasonality and genetic diversity of HMPV in a large outpatient population presented with ILI in Senegal between 2012 and 2016. Globally our results show a clear seasonal circulation pattern of HMPV in Senegal. Our findings identified children less than 5 years as more susceptible group to HMPV infection. Molecular studies identified A2, B1 and B2 as the major genotypes circulating.
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- 2019
- Full Text
- View/download PDF
4. Upper respiratory infections in a rural area with reduced malaria transmission in Senegal: a pathogens community study
- Author
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Roger C. Tine, Léon A. Ndiaye, Mbayame N. Niang, Davy E. Kiori, Ndongo Dia, Oumar Gaye, and Hélène Broutin
- Subjects
ARI ,Senegal ,Pathogen community ,Streptococcus pneumoniae ,RSV ,Influenza ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Acute Respiratory Infections (ARI) are common causes of febrile illnesses in many settings in Senegal. These infections are usually managed presumptively due to lack of appropriate diagnostic tools. This situation, can lead to poor management of febrile illness or antibiotic misuse. In addition, there are limited data on the spectrum of pathogens commonly responsible for these ARI. This study was conducted to explore the pathogens community among patients with acute respiratory infection in a rural area in Senegal. Methods A cross sectional study was conducted from August to December 2015. Children and adult patients attending Keur Socé health post for signs suggestive of acute respiratory infection were enrolled after providing inform consent. Eligible participants were recruited using a consecutive sampling method. Paired nose and throat swabs were collected for pathogen detection. Samples were processed using a multiplex PCR designed to identify 21 pathogens including both virus and bacteria. Results Two hundred and fifty patients participated in the study. Samples positivity rate was evaluated at 95.2% (238/250). Streptococcus pneumoniae was the predominant pathogen (74%) and was present in all months and all age-groups, followed by Staphylococcus aureus (28,8%) and rhinovirus (28,4%). Respiratory syncytial virus (RSV) was detected only among children under 5 years old in August and September while coronavirus was present in all age groups, during the months of October and December. Conclusion This pilot study revealed a diversity of pathogens over the time and across all age groups, highlighting the need for further exploration. A pathogen community approach including both virus and bacteria at a larger scale becomes crucial for a better understanding of transmission dynamics at population level in order to help shape ARI control strategies.
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- 2018
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5. Performance of case definitions and clinical predictors for influenza surveillance among patients followed in a rural cohort in Senegal
- Author
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Barry, Mamadou Aliou, primary, Arinal, Florent, additional, Talla, Cheikh, additional, Hedible, Boris Gildas, additional, Sarr, Fatoumata Diene, additional, Ba, Ibrahim Oumar, additional, Diop, Boly, additional, Dia, Ndongo, additional, and Vray, Muriel, additional
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- 2021
- Full Text
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6. Epidemiological, clinical and genotypic features of human Metapneumovirus in patients with influenza-like illness in Senegal, 2012 to 2016
- Author
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Mbayame Ndiaye Niang, Amary Fall, Davy Evrard Kiori, Ndongo Dia, Mamadou Malado Jallow, Malick Fall, Sara Sy, Mamadou Aliou Barry, and Déborah Goudiaby
- Subjects
0301 basic medicine ,Male ,Epidemiology ,viruses ,Pilot Projects ,medicine.disease_cause ,0302 clinical medicine ,Outpatients ,Influenza-like illness ,Prevalence ,030212 general & internal medicine ,Child ,Respiratory Tract Infections ,Phylogeny ,Aged, 80 and over ,Reactive airway disease ,education.field_of_study ,Paramyxoviridae Infections ,biology ,Coinfection ,Respiratory infection ,virus diseases ,Middle Aged ,Senegal ,Infectious Diseases ,Child, Preschool ,Bronchitis ,Female ,Rhinovirus ,Research Article ,Adult ,medicine.medical_specialty ,Adolescent ,Genotype ,030106 microbiology ,Population ,lcsh:Infectious and parasitic diseases ,03 medical and health sciences ,Human metapneumovirus ,Internal medicine ,Influenza, Human ,medicine ,Humans ,lcsh:RC109-216 ,education ,Aged ,Retrospective Studies ,business.industry ,Infant ,medicine.disease ,biology.organism_classification ,respiratory tract diseases ,Bronchiolitis ,Metapneumovirus ,business - Abstract
Background Human metapneumovirus (HMPV) is a causal agent of acute respiratory infection, especially in primarily children. At the clinical level, HMPV is associated to several diseases including bronchitis, croup, pneumonia, bronchiolitis, reactive airway disease, chronic obstructive pulmonary disease and asthma exacerbations, specifically in children less than 5 years. Here, we carried out a retrospective pilot study, based on the processing of nasopharyngeal swabs, with a focus on the epidemiology and molecular characteristics of HMPV in Senegal. Methods This retrospective study was conducted from January 2012 to December 2016. Briefly, all outpatients presenting to healthcare sentinel sites were screened for surveillance enrollment and included if they met criteria for ILI. Naso-oropharyngeal swabs were collected from eligible participants. For viral respiratory pathogens detection, including HMPV, the Anyplex™ II RV16 Detection kit was used. A fragment of the hMPV F gene was targeted for sequencing. Results In total, 8209 patients with ILI were enrolled. Half of them (49.7%) were children under 5 years. Fever was the most common symptom followed by cough, and rhinitis. Three hundred eight patients were positive for HMPV (3.75%). 89 (28.9%) were detected as single infection. In co-infection cases, the most common co-infecting viruses were influenza, adenovirus and rhinovirus. HMPV detection rates in the different age groups varied significantly with the children under 5 years group accounting for 71.7% of positive patients. The temporal distribution pattern for HMPV infection showed a clear seasonal pattern with a higher activity during the rainy period (July–September). Phylogenetic analyses revealed that HMPV specimens circulating in Senegal were distributed into the two main genetic lineages, A and B. We also noted a co-circulation of both genetic lineages during the whole study period except in 2014. Conclusion In summary, the present study characterized the recent prevalence, seasonality and genetic diversity of HMPV in a large outpatient population presented with ILI in Senegal between 2012 and 2016. Globally our results show a clear seasonal circulation pattern of HMPV in Senegal. Our findings identified children less than 5 years as more susceptible group to HMPV infection. Molecular studies identified A2, B1 and B2 as the major genotypes circulating.
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- 2019
7. Epidemiological, clinical and genotypic features of human Metapneumovirus in patients with influenza-like illness in Senegal, 2012 to 2016
- Author
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Jallow, Mamadou Malado, primary, Fall, Amary, additional, Kiori, Davy, additional, Sy, Sara, additional, Goudiaby, Déborah, additional, Barry, Mamadou Aliou, additional, Fall, Malick, additional, Niang, Mbayame Ndiaye, additional, and Dia, Ndongo, additional
- Published
- 2019
- Full Text
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8. Cytomegalovirus infection in HIV-infected versus non-infected infants and HIV disease progression in Cytomegalovirus infected versus non infected infants early treated with cART in the ANRS 12140—Pediacam study in Cameroon
- Author
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Josiane Warszawski, Patrice Tchendjou, Ida Calixte Penda, Paul Alain Ngoupo, Christine Rouzioux, Georgette Guemkam, Casimir Ledoux Sofeu, Francis Ateba Ndongo, Anfumbom Kfutwah, Mathurin Cyrille Tejiokem, Albert Faye, Suzie Tetang Ndiang, and Félicité Owona
- Subjects
0301 basic medicine ,Cart ,Male ,medicine.medical_specialty ,030106 microbiology ,Congenital cytomegalovirus infection ,Breastfeeding ,Cytomegalovirus ,HIV Infections ,03 medical and health sciences ,0302 clinical medicine ,Interquartile range ,Risk Factors ,Internal medicine ,medicine ,Prevalence ,Humans ,030212 general & internal medicine ,Cameroon ,Prospective Studies ,Prospective cohort study ,Developing Countries ,business.industry ,Coinfection ,Case-control study ,Infant, Newborn ,virus diseases ,Infant ,medicine.disease ,Early treated HIV-infected infants ,Co-infection ,Infectious Diseases ,Logistic Models ,Treatment Outcome ,Anti-Retroviral Agents ,Case-Control Studies ,Immunology ,Cytomegalovirus Infections ,Disease Progression ,Drug Therapy, Combination ,Female ,business ,Viral load ,Human Immunodeficiency Virus ,Research Article ,Follow-Up Studies - Abstract
Background The outcome of CMV/HIV co-infection in infants treated early with combined antiretroviral therapy (cART) in resource-limited settings has not been described. We aimed to estimate the prevalence and identify factors associated with early CMV infection in HIV-infected and non-infected infants included in a study in Cameroon, and to compare HIV disease progression and survival after 1 year of early cART, following infants’ CMV status. Methods HIV-infected infants followed from birth or from HIV diagnosis before 7 months old and HIV-uninfected infants born to HIV-infected or uninfected mothers were tested for CMV at a median age of 4.0 months [Interquartile range (IQR): 3.4–4.9]. Multivariable logistic regression was performed to identify factors associated with CMV infection. Early cART was offered to HIV-infected infants: mortality, immunological and virological outcomes were assessed. Results Three hundred and sixty-nine infants were tested. The proportion of infants infected with CMV at baseline was significantly higher in HIV-infected than in HIV-uninfected groups (58.9% (86/146) vs 30.0% (67/223), p
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- 2017
9. Upper respiratory infections in a rural area with reduced malaria transmission in Senegal: a pathogens community study
- Author
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Tine, Roger C., primary, Ndiaye, Léon A., additional, Niang, Mbayame N., additional, Kiori, Davy E., additional, Dia, Ndongo, additional, Gaye, Oumar, additional, and Broutin, Hélène, additional
- Published
- 2018
- Full Text
- View/download PDF
10. Cytomegalovirus infection in HIV-infected versus non-infected infants and HIV disease progression in Cytomegalovirus infected versus non infected infants early treated with cART in the ANRS 12140—Pediacam study in Cameroon
- Author
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Kfutwah, Anfumbom K. W., primary, Ngoupo, Paul Alain T., additional, Sofeu, Casimir Ledoux, additional, Ndongo, Francis Ateba, additional, Guemkam, Georgette, additional, Ndiang, Suzie Tetang, additional, Owona, Félicité, additional, Penda, Ida Calixte, additional, Tchendjou, Patrice, additional, Rouzioux, Christine, additional, Warszawski, Josiane, additional, Faye, Albert, additional, and Tejiokem, Mathurin Cyrille, additional
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- 2017
- Full Text
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11. Spread of Influenza A(H1N1) oseltamivir-resistant viruses in Africa in 2008 confirmed by multiple introductions in Senegal
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Déborah Goudiaby, Ndongo Dia, Saadiya A Diadhiou, Abdourahmane Faye, Mady Bâ, Ousmane M. Diop, Davy Evrard Kiori, R. Michel, and Mbayame Ndiaye Niang
- Subjects
Adult ,Oseltamivir ,Adolescent ,viruses ,Resistance ,Hemagglutinin Glycoproteins, Influenza Virus ,Microbial Sensitivity Tests ,Drug resistance ,medicine.disease_cause ,Antiviral Agents ,H5N1 genetic structure ,chemistry.chemical_compound ,Influenza A Virus, H1N1 Subtype ,Drug Resistance, Viral ,Influenza, Human ,Influenza A virus ,medicine ,Humans ,Child ,Gene ,Phylogeny ,biology ,Phylogenetic tree ,Reverse Transcriptase Polymerase Chain Reaction ,Infant ,virus diseases ,Middle Aged ,Virology ,Influenza ,Senegal ,Infectious Diseases ,chemistry ,Child, Preschool ,biology.protein ,Restriction fragment length polymorphism ,Neuraminidase ,Research Article - Abstract
Background Among Influenza neuraminidase inhibitors (NAIs), oseltamivir corresponds to the most widely used agent to treat influenza disease. However since 2001, several cases of resistance to NAIs have been reported for circulating seasonal A(H1N1) Influenza viruses. A direct resistance mechanism may be invoked, involving critical mutations in the viral NA gene that prevent the drug binding to its target. Same phenomenon is reported for adamantanes drugs and mutations in the M2 channel protein gene of Influenza viruses. Methods Reverse-Transcription/Restriction Fragment Length Polymorphism (RT-PCR/RFLP) method, phenotypic testing for oseltamivir resistance, and sequencing of NA, HA and M2 genes were used in this study. Phylogenetic analyses were performed using BioEdit and Mega 5 softwares for alignment of sequences and phylogenetic trees building respectively. Results Using a simple RT-PCR/RFLP method, we found that the 86 seasonal A(H1N1) isolates from 2008 bear the oseltamivir resistance-associated mutation (H274Y) in the NA gene. In contrast all isolates isolated in Senegal in 2007 were sensitive to oseltamivir. These results were first confirmed by finding high IC50 values using a phenotypic testing for oseltamivir resistance, and secondly by sequencing the whole NA gene. Regarding M2 gene, no mutation associated to adamantanes resistance was characterized of the isolates. Conclusions The present work provides evidence of circulation of drug-resistant seasonal A(H1N1) viruses during the 2008 influenza season (July to September) in Senegal. The results are in favor of multiple introductions of oseltamivir resistant viruses (ORV) A(H1N1) in Senegal. Phylogenetic analyses of isolates with complete sequences of N1 and HA1 genes showed that they belong to clade 2B and suggest sequential introductions in Africa.
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- 2013
- Full Text
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12. Respiratory viruses associated with patients older than 50 years presenting with ILI in Senegal, 2009 to 2011
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Dia, Ndongo, primary, Richard, Vincent, additional, Kiori, Davy, additional, Cisse, El Hadj Abdoul Khadir, additional, Sarr, Fatoumata Diène, additional, Faye, Abdourahmane, additional, Goudiaby, Déborah G, additional, Diop, Ousmane M, additional, and Niang, Mbayame N, additional
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- 2014
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13. Spread of Influenza A(H1N1) oseltamivir-resistant viruses in Africa in 2008 confirmed by multiple introductions in Senegal
- Author
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Dia, Ndongo, primary, Niang, Mbayame N, additional, Diadhiou, Saadiya A, additional, Goudiaby, Déborah G, additional, Faye, Abdourahmane, additional, Kiori, Davy, additional, Bâ, Mady, additional, Michel, Rémy, additional, and Diop, Ousmane M, additional
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- 2013
- Full Text
- View/download PDF
14. Spread of Influenza A(H1N1) oseltamivir-resistant viruses in Africa in 2008 confirmed by multiple introductions in Senegal.
- Author
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Ndongo Dia, Niang, Mbayame N., Diadhiou, Saadiya A., Goudiaby, Déborah G., Faye, Abdourahmane, Kiori, Davy, Bâ, Mady, Michel, Rémy, and Diop, Ousmane M.
- Subjects
H5N1 Influenza ,INFECTIOUS disease transmission ,OSELTAMIVIR ,DRUG resistance in microorganisms ,NEURAMINIDASE ,CHEMICAL inhibitors - Abstract
Background: Among Influenza neuraminidase inhibitors (NAIs), oseltamivir corresponds to the most widely used agent to treat influenza disease. However since 2001, several cases of resistance to NAIs have been reported for circulating seasonal A(H1N1) Influenza viruses. A direct resistance mechanism may be invoked, involving critical mutations in the viral NA gene that prevent the drug binding to its target. Same phenomenon is reported for adamantanes drugs and mutations in the M2 channel protein gene of Influenza viruses. Methods: Reverse-Transcription/Restriction Fragment Length Polymorphism (RT-PCR/RFLP) method, phenotypic testing for oseltamivir resistance, and sequencing of NA, HA and M2 genes were used in this study. Phylogenetic analyses were performed using BioEdit and Mega 5 softwares for alignment of sequences and phylogenetic trees building respectively. Results: Using a simple RT-PCR/RFLP method, we found that the 86 seasonal A(H1N1) isolates from 2008 bear the oseltamivir resistance-associated mutation (H274Y) in the NA gene. In contrast all isolates isolated in Senegal in 2007 were sensitive to oseltamivir. These results were first confirmed by finding high IC50 values using a phenotypic testing for oseltamivir resistance, and secondly by sequencing the whole NA gene. Regarding M2 gene, no mutation associated to adamantanes resistance was characterized of the isolates Conclusions: The present work provides evidence of circulation of drug-resistant seasonal A(H1N1) viruses during the 2008 influenza season (July to September) in Senegal. The results are in favor of multiple introductions of oseltamivir resistant viruses (ORV) A(H1N1) in Senegal. Phylogenetic analyses of isolates with complete sequences of N1 and HA1 genes showed that they belong to clade 2B and suggest sequential introductions in Africa. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
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