1. MAPK cascade gene family in Camellia sinensis: In-silico identification, expression profiles and regulatory network analysis
- Author
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Ashutosh Pandey, Anurag P. Srivastava, G. Meher Unnati, Archita Chatterjee, Tamalika Kar, Trisha Biswas, Neelam Mishra, Ruchika Rajput, Abhirup Paul, and Srijita Basak
- Subjects
0106 biological sciences ,0301 basic medicine ,MAPK/ERK pathway ,lcsh:QH426-470 ,MAP Kinase Signaling System ,In silico ,lcsh:Biotechnology ,Computational biology ,MAPK cascade ,Mitogen-activated protein kinase kinase ,01 natural sciences ,Camellia sinensis ,03 medical and health sciences ,Gene Expression Regulation, Plant ,Expression profile ,Arabidopsis ,lcsh:TP248.13-248.65 ,Genetics ,Gene family ,Arabidopsis thaliana ,Gene Regulatory Networks ,Gene ,Plant Proteins ,biology ,Functional interaction network ,biology.organism_classification ,lcsh:Genetics ,030104 developmental biology ,In-silico ,Mitogen-Activated Protein Kinases ,010606 plant biology & botany ,Biotechnology ,Research Article - Abstract
Background Mitogen Activated Protein Kinase (MAPK) cascade is a fundamental pathway in organisms for signal transduction. Though it is well characterized in various plants, there is no systematic study of this cascade in tea. Result In this study, 5 genes of Mitogen Activated Protein Kinase Kinase (MKK) and 16 genes of Mitogen Activated Protein Kinase (MPK) in Camellia sinensis were found through a genome-wide search taking Arabidopsis thaliana as the reference genome. Also, phylogenetic relationships along with structural analysis which includes gene structure, location as well as protein conserved motifs and domains, were systematically examined and further, predictions were validated by the results. The plant species taken for comparative study clearly displayed segmental duplication, which was a significant candidate for MAPK cascade expansion. Also, functional interaction was carried out in C. sinensis based on the orthologous genes in Arabidopsis. The expression profiles linked to various stress treatments revealed wide involvement of MAPK and MAPKK genes from Tea in response to various abiotic factors. In addition, the expression of these genes was analysed in various tissues. Conclusion This study provides the targets for further comprehensive identification, functional study, and also contributed for a better understanding of the MAPK cascade regulatory network in C. sinensis.
- Published
- 2020
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