1. Effectiveness of 10 polymorphic microsatellite markers for parentage and pedigree analysis in plateau pika (Ochotona curzoniae)
- Author
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Yanming Zhang, Jiapeng Qu, Kexin Li, Jianing Geng, and Songnian Hu
- Subjects
Male ,lcsh:QH426-470 ,Genotype ,Ochotona curzoniae ,Population ,Genetics ,Polymorphic Microsatellite Marker ,Animals ,Genetics(clinical) ,Pika ,education ,Genetics (clinical) ,Lod score ,education.field_of_study ,geography ,Plateau ,geography.geographical_feature_category ,biology ,Lagomorpha ,Sequence Analysis, DNA ,biology.organism_classification ,Pedigree ,lcsh:Genetics ,Genetics, Population ,Evolutionary biology ,Genetic marker ,Microsatellite ,Female ,Lod Score ,Research Article ,Microsatellite Repeats - Abstract
Background The plateau pika (Ochotona curzoniae) is an underground-dwelling mammal, native to the Tibetan plateau of China. A set of 10 polymorphic microsatellite loci has been developed earlier. Its reliability for parentage assignment has been tested in a plateau pika population. Two family groups with a known pedigree were used to validate the power of this set of markers. Results The error in parentage assignment using a combination of these 10 loci was very low as indicated by their power of discrimination (0.803 - 0.932), power of exclusion (0.351 - 0.887), and an effectiveness of the combined probability of exclusion in parentage assignment of 99.999%. Conclusion All the offspring of a family could be assigned to their biological mother; and their father or relatives could also be identified. This set of markers therefore provides a powerful and efficient tool for parentage assignment and other population analyses in the plateau pika.
- Published
- 2010