1. Sp1 and KLF15 regulate basal transcription of the human LRP5 gene
- Author
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Yongxin Zou, Yaoqin Gong, Xiyu Zhang, Baichun Jiang, Jiangxia Li, and Yang Yang
- Subjects
Transcriptional Activation ,Chromatin Immunoprecipitation ,lcsh:QH426-470 ,Sp1 Transcription Factor ,Molecular Sequence Data ,Kruppel-Like Transcription Factors ,Biology ,Transcription (biology) ,Cell Line, Tumor ,Research article ,Transcriptional regulation ,Genetics ,Animals ,Humans ,Genetics(clinical) ,Cloning, Molecular ,Promoter Regions, Genetic ,Gene ,Transcription factor ,Genetics (clinical) ,LDL-Receptor Related Proteins ,Regulation of gene expression ,Sp1 transcription factor ,Binding Sites ,General transcription factor ,Base Sequence ,Nuclear Proteins ,Molecular biology ,lcsh:Genetics ,Low Density Lipoprotein Receptor-Related Protein-5 ,Gene Expression Regulation ,Drosophila ,Chromatin immunoprecipitation - Abstract
Background LRP5, a member of the low density lipoprotein receptor superfamily, regulates diverse developmental processes in embryogenesis and maintains physiological homeostasis in adult organisms. However, how the expression of human LRP5 gene is regulated remains unclear. Results In order to characterize the transcriptional regulation of human LRP5 gene, we cloned the 5' flanking region and evaluated its transcriptional activity in a luciferase reporter system. We demonstrated that both KLF15 and Sp1 binding sites between -72 bp and -53 bp contribute to the transcriptional activation of human LRP5 promoter. Chromatin immunoprecipitation assay demonstrated that the ubiquitous transcription factors KLF15 and Sp1 bind to this region. Using Drosophila SL2 cells, we showed that KLF15 and Sp1 trans-activated the LRP5 promoter in a manner dependent on the presence of Sp1-binding and KLF15-binding motifs. Conclusions Both KLF15 and Sp1 binding sites contribute to the basal activity of human LRP5 promoter. This study provides the first insight into the mechanisms by which transcription of human LRP5 gene is regulated.
- Published
- 2010