1. Human placenta induces hair regrowth in chemotherapy-induced alopecia via inhibition of apoptotic factors and proliferation of hair follicles.
- Author
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Kim, Mi Hye, Kim, Kyuseok, Lee, Haesu, and Yang, Woong Mo
- Subjects
CELL proliferation ,ANIMAL experimentation ,APOPTOSIS ,BALDNESS ,CANCER chemotherapy ,GENE expression ,HAIR follicles ,HEMOPROTEINS ,HISTOLOGICAL techniques ,INTRAPERITONEAL injections ,ONCOGENES ,PHOSPHORYLATION ,PLACENTA ,PROTEIN kinases ,RATS ,SKIN ,STAINS & staining (Microscopy) ,CUTANEOUS therapeutics ,TISSUE extracts ,NUCLEAR proteins ,CYCLOPHOSPHAMIDE ,CASPASES - Abstract
Background: The human placenta (HP) is a complex organ used to alleviate tiredness and promote wound healing. Previous research showed the hair growth-promoting effect of HP. However, no reports have addressed the effects of HP on hair regrowth in chemotherapy-induced alopecia. In this study, we investigated the effects of HP on the apoptosis and proliferation of hair follicles in chemotherapy-induced alopecia. Methods: Male C57BL/6 mice in telogen were depilated to enter anagen. After 9 days, dystrophic catagen was induced by the intraperitoneal injection of 150 mg/kg cyclophosphamide. During 9 to 16 days, 0.1 and 1 mg/mL HP were topically applied to depilated dorsal skin. Results: Dystrophic hair follicles by cyclophosphamide were recovered by HP treatment. New hair shafts containing hair fibers appeared to be straight after HP treatment. Immunohistological staining revealed a significant increase of Ki67-positive cells in hair follicles treated with 1 mg/mL HP. Topical HP treatment increased the ratio of Bcl-2/Bax, while it attenuated the expression of pro-apoptotic Bax, p53, and cytochrome c with caspase-9 and -3. In addition, the expression of KGF and the phosphorylation of AKT were upregulated by HP treatment. Conclusion: These results suggest that HP treatment induced hair growth by inhibiting apoptosis and promoting the proliferation of hair follicles. HP may be useful for treating chemotherapy-induced alopecia. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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